Park Jin-Young, Lee Jung-Hee, Kim Chun-Ho, Kim Young-Jin
Department of Biomedical Engineering, Daegu Catholic University Gyeongsan 38430 Republic of Korea
Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences Seoul 01812 Republic of Korea.
RSC Adv. 2018 Oct 8;8(60):34359-34369. doi: 10.1039/c8ra05637d. eCollection 2018 Oct 4.
In this study, we first prepared the precursor polytetrafluoroethylene (PTFE)/poly(ethylene oxide) (PEO) nanofibrous membranes by electrospinning with different PTFE/PEO weight ratios. These membranes exhibited three-dimensional interconnected pore structures. The average diameter of the precursor nanofibres decreased with increased PTFE contents from 633 ± 34 nm (PTFE/PEO weight ratio of 5 : 1) to 555 ± 63 nm (PTFE/PEO weight ratio of 7 : 1) because of the decrease in solution viscosity. Then, the precursor membranes were sintered with different temperatures to obtain the PTFE nanofibrous membranes, resulting in the average diameter of the nanofibres increasing from 633 ± 34 nm to 947 ± 78 nm with the increase in sintering temperature; consequently, the membrane became more compact. This compaction caused a decrease in porosity from 76.5 ± 2.9% to 69.1 ± 2.6% and an increase in water contact angle from 94.1 ± 4.2° to 143.3 ± 3.5°. In addition, the mechanical properties of the PTFE nanofibrous membranes increased with increasing sintering temperature. Cytocompatibility test results revealed that the PTFE350 membrane, which was sintered at 350 °C, promoted the proliferation and differentiation of MC3T3-E1 cells more rapidly than other membrane types. These results suggested that the PTFE nanofibrous membranes could be ideal biomaterials in tissue engineering for bone regeneration.
在本研究中，我们首先通过静电纺丝制备了具有不同聚四氟乙烯（PTFE）/聚环氧乙烷（PEO）重量比的前驱体聚四氟乙烯（PTFE）/聚环氧乙烷（PEO）纳米纤维膜。这些膜呈现出三维相互连接的孔结构。由于溶液粘度降低，前驱体纳米纤维的平均直径随着PTFE含量的增加从633±34 nm（PTFE/PEO重量比为5∶1）降至555±63 nm（PTFE/PEO重量比为7∶1）。然后，将前驱体膜在不同温度下烧结以获得PTFE纳米纤维膜，随着烧结温度的升高，纳米纤维的平均直径从633±34 nm增加到947±78 nm；因此，膜变得更加致密。这种致密化导致孔隙率从76.5±2.9%降至69.1±2.6%，水接触角从94.1±4.2°增加到143.3±3.5°。此外，PTFE纳米纤维膜的力学性能随着烧结温度的升高而增强。细胞相容性测试结果表明，在350℃烧结的PTFE350膜比其他膜类型更能快速促进MC3T3-E1细胞的增殖和分化。这些结果表明，PTFE纳米纤维膜可能是骨再生组织工程中的理想生物材料。
