Xu ChangJun, Ge HaiTao, Wang Tao, Qin JianBing, Liu De, Liu YuGuang
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Department of Neurosurgery, Shangdong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
J Stroke Cerebrovasc Dis. 2018 May;27(5):1226-1236. doi: 10.1016/j.jstrokecerebrovasdis.2017.11.041. Epub 2018 Jan 5.
To study the expression of T cell immunoglobulin and mucin domain 3 (Tim-3) on peripheral blood immunocytes, and the relationship between Tim-3 and the systemic inflammatory response or brain injury in patients with intracerebral hemorrhage (ICH).
According to the volume of hematoma at 12 hours after onset of ICH, 60 newly diagnosed patients with ICH were divided into the small (volume of hematoma <30 mL, 30 cases) and large (volume of hematoma ≥30 mL, 30 cases) ICH groups. The expression of Tim-3 on peripheral blood immunocytes was analyzed by flow cytometry. Real-time reverse transcriptase polymerase chain reaction was used to detect Tim-3 mRNA on peripheral blood mononuclear cells (PBMCs). Meanwhile, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and S-100B protein were measured by enzyme-linked immunosorbent assay. Glasgow outcome scale (GOS) score at Day 30 was used to estimate prognosis of patients.
The leukocyte count, neutrophil count, monocyte count, TNF-α, IL-1β, and S-100B protein increased remarkably after ICH. However, all of them in the large ICH group increased more obviously, and there were significant differences when compared with those in the small ICH group (P < .01). The expression of Tim-3 mRNA on PBMCs in the large ICH group increased remarkably, peaked at Day 3, and was positively associated with the concentrations of TNF-α, IL-1β, and S-100B protein (P < .01). Tim-3 was predominantly expressed itself on CD14 monocytes. There was a negative correlation between GOS score and Tim-3 mRNA, TNF-α, IL-1β, or S-100B protein.
The expression of Tim-3 on CD14 + monocytes involves in systemic inflammatory reaction after ICH and may be a novel treatment target.
研究T细胞免疫球蛋白黏蛋白结构域3（Tim-3）在外周血免疫细胞上的表达，以及脑出血（ICH）患者中Tim-3与全身炎症反应或脑损伤之间的关系。
根据ICH发病后12小时血肿体积，将60例新诊断的ICH患者分为小血肿组（血肿体积＜30 mL，30例）和大血肿组（血肿体积≥30 mL，30例）。采用流式细胞术分析外周血免疫细胞上Tim-3的表达。应用实时逆转录聚合酶链反应检测外周血单个核细胞（PBMCs）上Tim-3 mRNA。同时，采用酶联免疫吸附测定法检测肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和S-100B蛋白。采用格拉斯哥预后量表（GOS）评分评估患者30天时的预后。
ICH后白细胞计数、中性粒细胞计数、单核细胞计数、TNF-α、IL-1β和S-100B蛋白显著升高。然而，大血肿组上述指标升高更明显，与小血肿组相比差异有统计学意义（P＜0.01）。大血肿组PBMCs上Tim-3 mRNA表达显著升高，在第3天达到峰值，且与TNF-α、IL-1β和S-100B蛋白浓度呈正相关（P＜0.01）。Tim-3主要在CD14单核细胞上表达。GOS评分与Tim-3 mRNA、TNF-α、IL-1β或S-100B蛋白之间呈负相关。
CD14⁺单核细胞上Tim-3的表达参与了ICH后的全身炎症反应，可能是一个新的治疗靶点。
