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四个分离株的基因组及对该物种复合体的新见解。

The Genomes of Four Isolates and Novel Insights into the Species Complex.

作者信息

De Marco Leone, Epis Sara, Capone Aida, Martin Elena, Bozic Jovana, Crotti Elena, Ricci Irene, Sassera Davide

机构信息

School of Bioscience and Veterinary Medicine, University of Camerino, 62032, Italy.

Department of Biology and Biotechnology, University of Pavia, 27100, Italy.

出版信息

G3 (Bethesda). 2018 Mar 2;8(3):755-759. doi: 10.1534/g3.117.300316.

Abstract

Yeasts of the species complex are widespread in nature and can be isolated from a variety of sources, from the environment to arthropods to hospital patients. To date, the species complex comprises the thoroughly studied and versatile , the hard to distinguish , and Here we report the whole genome sequencing and assembly of four isolates, identified with the most recent molecular techniques, derived from four Diptera species. The four novel assemblies present reduced fragmentation and comparable metrics (genome size, gene content) to the available genomes belonging to the species complex. We performed a phylogenomic analysis comprising all known members of the species complex, to investigate evolutionary relationships within this clade. Our results show a compact phylogenetic structure for the complex and indicate the presence of a sizable core set of genes. Furthermore, , despite a broad literature on the difficulties of discerning it from , seems to be more closely related to Finally, we believe that there is evidence for considering these four genomes to be the first published for the species Raw reads and assembled contigs have been made public to further the study of these organisms.

摘要

该物种复合体的酵母在自然界中广泛存在,可从多种来源分离得到,从环境到节肢动物再到医院患者。迄今为止,该物种复合体包括经过充分研究且用途广泛的[物种名称1]、难以区分的[物种名称2]以及[物种名称3]。在此,我们报告了通过最新分子技术鉴定的、源自四种双翅目物种的四个[物种名称]分离株的全基因组测序和组装情况。这四个新的组装体片段化程度降低,与该物种复合体中现有基因组的指标(基因组大小、基因含量)相当。我们进行了系统发育基因组学分析,涵盖该物种复合体的所有已知成员,以研究这一进化枝内的进化关系。我们的结果显示该复合体具有紧凑的系统发育结构,并表明存在一组相当数量的核心基因。此外,尽管有大量文献论述了将[物种名称4]与[物种名称5]区分开来的困难,但[物种名称4]似乎与[物种名称6]关系更为密切。最后,我们认为有证据表明可将这四个基因组视为首次发表的[物种名称7]的基因组。原始读数和组装的重叠群已公开,以推动对这些生物体的进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fbd/5844296/990bee98d9d1/755f1.jpg

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