Valsalan Ravisankar, Mathew Deepu
Bioinformatics Centre, Kerala Agricultural University, KAU Post, Thrissur, Kerala State, 680 656, India.
J Genet Eng Biotechnol. 2020 Sep 29;18(1):54. doi: 10.1186/s43141-020-00074-2.
Meyerozyma guilliermondii is a yeast which could be isolated from a variety of environments. The vka1 strain isolated and purified from the organic compost was found to have composting potential. To better understand the genes assisting the composting potential in this yeast, whole genome sequencing and sequence annotation were performed.
The genome of M. guilliermondii vka1 strain was sequenced using a hybrid approach, on Illumina Hiseq-2500 platform at 100× coverage followed by Nanopore platform at 20× coverage. The de novo assembly using dual-fold approach had given draft genome of 10.8 Mb size. The genome was found to contain 5385 genes. The annotation of the genes was performed, and the enzymes identified to have roles in the degradation of macromolecules are discussed in relation to its composting potential. Annotation of the genome assembly of the related strains had revealed the unique biodegradation related genes in this strain. Phylogenetic analysis using the rDNA region has confirmed the position of this strain in the Ascomycota family. Raw reads are made public, and the genome wide proteome profile is presented to facilitate further studies on this organism.
Meyerozyma guilliermondii vka1 strain was sequenced through hybrid approach and the reads were de novo assembled. Draft genome size and the number of genes in the strain were assessed and discussed in relation to the related strains. Scientific insights into the composting potential of this strain are also presented in relation to the unique genes identified in this strain.
季也蒙毕赤酵母是一种可从多种环境中分离得到的酵母。从有机堆肥中分离纯化得到的vka1菌株被发现具有堆肥潜力。为了更好地了解该酵母中有助于堆肥潜力的基因，进行了全基因组测序和序列注释。
采用混合方法对季也蒙毕赤酵母vka1菌株的基因组进行测序，先在Illumina Hiseq - 2500平台上进行100倍覆盖度测序，随后在Nanopore平台上进行20倍覆盖度测序。使用双重方法进行的从头组装得到了大小为10.8 Mb的基因组草图。发现该基因组包含5385个基因。对这些基因进行了注释，并讨论了鉴定出的在大分子降解中起作用的酶与其堆肥潜力的关系。对相关菌株基因组组装的注释揭示了该菌株中独特的生物降解相关基因。使用rDNA区域进行的系统发育分析证实了该菌株在子囊菌科中的位置。原始读数已公开，并呈现了全基因组蛋白质组概况，以促进对该生物体的进一步研究。
通过混合方法对季也蒙毕赤酵母vka1菌株进行了测序，并对读数进行了从头组装。评估并讨论了该菌株的基因组草图大小和基因数量与相关菌株的关系。还结合该菌株中鉴定出的独特基因，对该菌株的堆肥潜力进行了科学分析。
