Morais Sebastião Vieira de, Czeczko Nicolau Gregori, Malafaia Osvaldo, Ribas Jurandir Marcondes, Garcia João Batista Santos, Miguel Marcelo Tizzot, Zini Cassio, Massignan Angeline Garcez
Fellow Master degree, Postgraduate Program in Principles of Surgery, Faculdade Evangélica do Paraná (FEPAR), Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Conception, design, intellectual and scientific content of the study.
Associate Professor, Postgraduate Program in Principles of Surgery, FEPAR, Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Scientific and intellectual content of the study, interpretation of data, critical revision.
Acta Cir Bras. 2016 Nov;31(11):765-773. doi: 10.1590/S0102-865020160110000010.
: To evaluate the usefulness of a knee osteoarthritis model through functional, radiological and microscopic changes of the synovial membrane.
: Forty eight rats were divided randomly into two groups. The first received 0.9% saline in the joint and corresponded to the control group. The second was submitted to experimental osteoarthritis of the right knee induced by monosodium iodoacetate and corresponded to the osteoarthritis group. All animals were subjected to comparative tests of forced ambulation and joint movements, inability to articulate and tactile allodynia on day 1 post-experiment by forced ambulation (Roto-rod test), joint assessment of disability (weight bearing test) and assessment of tactile allodynia (Von Frey test). After inflammatory induction they were divided into four sub-groups corresponding to the scheduled death in 7, 14, 21 and 28 days when they were submitted to radiographic examination of the knee, arthrotomy and collection of the synovial membrane.
: The osteoarthritis group showed significant differences compared to control group on days 7 and 14 in Roto-rod, in weight bearing and Von Frey tests in all days, and in radiological evaluation. Microscopic examination of the synovial membrane showed abnormalities of inflammatory character at all stages.
: The osteoarthritis induced by intra-articular monosodium iodoacetate in rats knee is a good model to be used in related research, because it provides mensurable changes on joint movements, tactile allodynia, progressive radiological degeneration and microscopic inflammation of the synovial membrane, that represent markers for osteoarthritis evaluation.
通过滑膜的功能、放射学和微观变化评估膝骨关节炎模型的效用。
48只大鼠随机分为两组。第一组在关节内注射0.9%生理盐水，为对照组。第二组通过注射碘乙酸钠诱导右膝实验性骨关节炎，为骨关节炎组。所有动物在实验后第1天接受强迫行走和关节活动的对比测试、关节活动障碍（负重测试）和触觉异常性疼痛评估（von Frey测试）。炎症诱导后，根据预定在7、14、21和28天处死将它们分为四个亚组，处死时对其进行膝关节X线检查、关节切开术并收集滑膜。
骨关节炎组在第7天和第14天的旋转棒测试、所有天数的负重和von Frey测试以及放射学评估方面与对照组相比有显著差异。滑膜的微观检查显示在所有阶段均有炎症特征的异常。
大鼠膝关节内注射碘乙酸钠诱导的骨关节炎是用于相关研究的良好模型，因为它在关节活动、触觉异常性疼痛、渐进性放射学退变和滑膜微观炎症方面提供了可测量的变化，这些变化是骨关节炎评估的标志物。
