Buscher Konrad, Marcovecchio Paola, Hedrick Catherine C, Ley Klaus
Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA, United States.
Department of Internal Medicine, Nephrology and Rheumatology, University Hospital Münster, Münster, Germany.
Front Cardiovasc Med. 2017 Dec 19;4:80. doi: 10.3389/fcvm.2017.00080. eCollection 2017.
Non-classical monocytes have emerged as the preeminent vascular housekeepers. Continuous intravascular screening is enabled by slow patrolling on the endothelium and allows a rapid response to local perturbations. Intravital imaging has been crucial to elucidate the molecular mechanisms and migratory phenotype of patrolling. In this review, we discuss technical requirements of intravital microscopy such as imaging modalities, labeling strategies, and data analysis. We further focus on patrolling kinetics and adhesion receptors in different organs and vascular beds including arteries during homeostasis and vascular inflammation and define pertinent questions in the field.
非经典单核细胞已成为卓越的血管守护者。通过在内皮上缓慢巡逻实现持续的血管内筛查，并能对局部扰动做出快速反应。活体成像对于阐明巡逻的分子机制和迁移表型至关重要。在本综述中，我们讨论了活体显微镜检查的技术要求，如成像方式、标记策略和数据分析。我们进一步关注不同器官和血管床（包括内稳态和血管炎症期间的动脉）中的巡逻动力学和黏附受体，并确定该领域的相关问题。
