溶菌性分枝杆菌噬菌体D29对结核分枝杆菌感染的巨噬细胞RAW 264细胞系模型的效率评估。
Evaluation of the Efficiency of Lytic Mycobacteriophage D29 on the Model of M. tuberculosis-Infected Macrophage RAW 264 Cell Line.
作者信息
Lapenkova M B, Smirnova N S, Rutkevich P N, Vladimirsky M A
机构信息
Research Institute of Phthisiopulmonology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
Research Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2018 Jan;164(3):344-346. doi: 10.1007/s10517-018-3986-0. Epub 2018 Jan 8.
Culture of mouse macrophages (RAW 264.7 ATCC strain) in wells of a 6-well plate was infected with M. tuberculosis in proportion of 15 mycobacteria per one macrophage and then treated with a lytic strain of mycobacteriophage D29. Antibacterial efficacy of mycobacteriophages was studied using D29 phage (activity 108 plaque-forming units/ml) previously purified by ion exchange chromatography. After single and double 24-h treatment, the lysed cultures of macrophages were inoculated onto Middlebrook 7H10 agar medium. The number of mycobacterial colonies in control and test wells (at least 3 wells in each group) was 300.178±12.500 and 36.0±5.4, respectively (p<0.01).
将小鼠巨噬细胞（RAW 264.7 ATCC菌株）接种于6孔板的孔中，以每一个巨噬细胞对应15个分枝杆菌的比例感染结核分枝杆菌，然后用裂解性分枝杆菌噬菌体D29进行处理。使用先前通过离子交换色谱法纯化的D29噬菌体（活性为108噬菌斑形成单位/毫升）研究分枝杆菌噬菌体的抗菌效果。在进行单次和两次24小时处理后，将裂解的巨噬细胞培养物接种到Middlebrook 7H10琼脂培养基上。对照孔和测试孔（每组至少3个孔）中的分枝杆菌菌落数分别为300.178±12.500和36.0±5.4（p<0.01）。
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