Section for Infection Medicine, Department of Clinical Sciences Lund, Lund University, Skane University Hospital, Lund, Sweden.
Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Am J Trop Med Hyg. 2018 Mar;98(3):791-796. doi: 10.4269/ajtmh.17-0692. Epub 2018 Jan 4.
Childhood vaccination with the 13-valent pneumococcal conjugate vaccine (PCV13) was introduced in Cambodia in January 2015. Baseline data regarding circulating serotypes are scarce. All microbiology laboratories in Cambodia were contacted for identification of stored isolates of from clinical specimens taken before the introduction of PCV13. Available isolates were serotyped using a multiplex polymerase chain reaction method. Among 166 identified isolates available for serotyping from patients with pneumococcal disease, 4% were isolated from upper respiratory samples and 80% were from lower respiratory samples, and 16% were invasive isolates. PCV13 serotypes accounted for 60% (95% confidence interval [CI] 52-67) of all isolates; 56% (95% CI 48-64) of noninvasive and 77% (95% CI 57-89) of invasive isolates. Antibiotic resistance was more common among PCV13 serotypes. This study of clinical isolates supports the potential for high reduction in pneumococcal disease burden and may serve as baseline data for future monitoring of serotypes circulation after implementation of PCV13 childhood vaccination in Cambodia.
2015 年 1 月，柬埔寨开始为儿童接种 13 价肺炎球菌结合疫苗（PCV13）。目前有关循环血清型的数据十分有限。我们联系了柬埔寨所有的微生物学实验室，以鉴定在 PCV13 引入之前，从临床标本中分离出的肺炎链球菌。采用多重聚合酶链反应方法对可用的分离株进行血清分型。在可用于对肺炎球菌疾病患者进行血清分型的 166 株分离株中，4%分离自上呼吸道样本，80%分离自下呼吸道样本，16%为侵袭性分离株。PCV13 血清型占所有分离株的 60%（95%置信区间[CI]52-67）；非侵袭性分离株中 56%（95% CI 48-64）和侵袭性分离株中 77%（95% CI 57-89）为 PCV13 血清型。PCV13 血清型的抗生素耐药性更为常见。本项针对临床分离株的研究支持高比例降低肺炎球菌疾病负担的潜力，并且可能成为柬埔寨实施 PCV13 儿童免疫接种后监测肺炎链球菌血清型循环的基线数据。
