Trejdosiewicz L K, Malizia G, Oakes J, Losowsky M S, Janossy G
J Clin Pathol. 1985 Sep;38(9):1002-6. doi: 10.1136/jcp.38.9.1002.
Expression of the gp100 common acute lymphoblastic leukaemia antigen (CALLA) was studied in the mucosa of the gut by means of indirect immunofluorescence on cryostat tissue sections with a panel of eight monoclonal antibodies to common acute lymphoblastic leukaemia antigen (anti-CALLA antibodies) and two antibodies to non-CALLA leukaemic antigens. Expression of CALLA was absent from normal stomach epithelium, adult and fetal colonic epithelium of normal histology, and colonic epithelium from patients with Crohn's disease or ulcerative colitis. By contrast, all eight anti-CALLA antibodies gave a characteristic reaction in normal adult and fetal small bowel mucosa, with specific localisation to the entire brush border of jejunal epithelium. Whereas seven of these antibodies reacted both with normal jejunal epithelium and with the damaged epithelium of patients with coeliac disease, antibody RFAL-2 reacted strongly only with histologically normal small bowel but more weakly in patients with coeliac disease to a degree related to the amount of histological abnormality. Expression of the moeity like CALLA identified with RFAL-2 was strongest in crypt epithelium and proportionally diminished along the villi according to the amount of histological damage in coeliac disease, being essentially absent in patients with "subtotal villous atrophy."
利用一组针对常见急性淋巴细胞白血病抗原（抗 CALLA 抗体）的八种单克隆抗体以及两种针对非 CALLA 白血病抗原的抗体，通过对低温恒温器组织切片进行间接免疫荧光法，研究了肠道黏膜中 gp100 常见急性淋巴细胞白血病抗原（CALLA）的表达情况。正常胃上皮、组织学正常的成人及胎儿结肠上皮以及克罗恩病或溃疡性结肠炎患者的结肠上皮均未检测到 CALLA 的表达。相比之下，所有八种抗 CALLA 抗体在正常成人及胎儿小肠黏膜中均呈现特征性反应，特异性定位于空肠上皮的整个刷状缘。其中七种抗体既与正常空肠上皮反应，也与乳糜泻患者受损上皮反应，而抗体 RFAL - 2 仅与组织学正常的小肠强烈反应，在乳糜泻患者中反应较弱，其程度与组织学异常程度相关。用 RFAL - 2 鉴定的类似 CALLA 成分的表达在隐窝上皮中最强，并根据乳糜泻中的组织学损伤程度沿绒毛按比例减少，在“完全绒毛萎缩”患者中基本不存在。
