Metzgar R S, Borowitz M J, Jones N H, Dowell B L
J Exp Med. 1981 Oct 1;154(4):1249-54. doi: 10.1084/jem.154.4.1249.
The common acute lymphoblastic leukemia antigen (CALLA), as defined by J-5 murine monoclonal antibodies, was detected on renal tubular and glomerular cells from fetal and adult donors by an indirect immunoperoxidase technique. CALLA could also be detected on epithelial cells of the fetal small intestine and on myoepithelial cells of adult breast but not on myoepithelial cells of the salivary gland. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of immunoprecipitated 125I-labeled membrane antigens from dissociated renal cells demonstrated that the antigen migrated as a 90,000 mol wt antigen rather than the 98,000-100,000 mol wt antigen noted on CALLA-positive tissue culture cell lines. The data suggest that the determinant defined by the J-5 monoclonal antibody is neither a lymphoid cell-specific differentiation antigen nor a leukemia-specific antigen.
用J-5鼠单克隆抗体所界定的常见急性淋巴细胞白血病抗原（CALLA），通过间接免疫过氧化物酶技术在来自胎儿和成年供体的肾小管和肾小球细胞上被检测到。CALLA也能在胎儿小肠的上皮细胞以及成年乳腺的肌上皮细胞上被检测到，但在唾液腺的肌上皮细胞上未被检测到。对来自解离肾细胞的免疫沉淀的125I标记膜抗原进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，该抗原迁移为一种90,000道尔顿分子量的抗原，而非在CALLA阳性组织培养细胞系上所观察到的98,000 - 100,000道尔顿分子量的抗原。数据表明，由J-5单克隆抗体所界定的决定簇既不是淋巴细胞特异性分化抗原，也不是白血病特异性抗原。
