Lebacq-Verheyden A M, Ravoet A M, Bazin H, Sutherland D R, Tidman N, Greaves M F
Int J Cancer. 1983 Sep 15;32(3):273-9. doi: 10.1002/ijc.2910320303.
Four distinct rat monoclonal antibodies against the common ALL antigen (CALLA, gp 100) were obtained in a single fusion. Rat AL2, AL3, AL4, AL5 and the previously reported mouse J5 monoclonal antibodies identified the same subsets of leukaemic cells. AL2 and AL3 reacted weakly with terminal transferase-positive cells in normal bone marrow and foetal liver, as well as with a minority of mature granulocytes in blood. Immunoprecipitation experiments and competitive binding assays demonstrated that the four rat antibodies and J5 bound to the same glycoprotein of approximately 100,000 mol. wt. This set of rat monoclonal antibodies directed against CALLA has not only a diagnostic usefulness but may also be of therapeutic value.
在一次融合中获得了四种针对常见急性淋巴细胞白血病抗原（CALLA，gp 100）的不同大鼠单克隆抗体。大鼠AL2、AL3、AL4、AL5以及先前报道的小鼠J5单克隆抗体识别相同的白血病细胞亚群。AL2和AL3与正常骨髓和胎肝中末端转移酶阳性细胞以及血液中少数成熟粒细胞反应较弱。免疫沉淀实验和竞争性结合试验表明，这四种大鼠抗体和J5与同一种分子量约为100,000的糖蛋白结合。这组针对CALLA的大鼠单克隆抗体不仅具有诊断用途，还可能具有治疗价值。
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