Toulmond A, Slitine F E I, De Frescheville J, Jouin C
Biol Bull. 1990 Dec;179(3):366-373. doi: 10.2307/1542329.
The polychaete annelids Alvinella pompejana, Alvinella caudata, and Paralvinella grasslei are strictly associated with deep sea hydrothermal vents. Each species possesses an extracellular hemoglobin, Hb, which has been studied and compared to that of a common intertidal polychaete, the lugworm Arenicola marina. The four Hbs exhibit very similar quaternary structures and spectral properties, and only small differences appeared in the gross polypeptide compositions after reduction and sodium dodecyl sulfate denaturation of the native molecules. Conversely, by a comparison of the effects of pH (6.6-7.6) and temperature (10-40{deg}C) on their intrinsic O affinities, Bohr factors, cooperativities, and apparent heats of oxygenation, lugworm Hb can be differentiated from that of the alvinellids, and the Hb of A. pompejana from that of A. caudata. The known biology of the lugworm and a further analysis of the data suggest several hypotheses concerning the in vivo O2 transport function of the alvinellid Hbs, the in vivo blood pH value in the two alvinellid species, their respective range of optimal temperature, and their ability to create a differentiated and stable external microenvironment.
多毛纲环节动物庞贝阿尔文蚓（Alvinella pompejana）、尾阿尔文蚓（Alvinella caudata）和格拉斯勒副阿尔文蚓（Paralvinella grasslei）与深海热液喷口紧密相关。每个物种都拥有一种细胞外血红蛋白（Hb），这种血红蛋白已被研究，并与常见的潮间带多毛纲动物沙蠋（Arenicola marina）的血红蛋白进行了比较。这四种血红蛋白表现出非常相似的四级结构和光谱特性，并且在天然分子还原和十二烷基硫酸钠变性后，其总多肽组成仅出现微小差异。相反，通过比较pH值（6.6 - 7.6）和温度（10 - 40℃）对它们内在氧亲和力、玻尔因子、协同性和表观氧合热的影响，可以区分沙蠋血红蛋白与阿尔文蚓血红蛋白，以及庞贝阿尔文蚓血红蛋白与尾阿尔文蚓血红蛋白。沙蠋已知的生物学特性以及对数据的进一步分析提出了几个假设，涉及阿尔文蚓血红蛋白在体内的氧气运输功能、两种阿尔文蚓物种体内的血液pH值、它们各自的最适温度范围以及它们创造差异化和稳定外部微环境的能力。
