Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Institute for Clinical Research, National Mie Hospital, Tsu, Mie, Japan.
J Med Virol. 2018 May;90(5):890-898. doi: 10.1002/jmv.25016. Epub 2018 Feb 5.
The emergence and rapid spread of novel DS-1-like intergenogroup reassortant rotaviruses having the equine-like G3 genotype (DS-1-like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015-2016, three DS-1-like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full-genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS-1-like G1P[8] and/or Japanese equine-like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3-VP7 gene from equine G3 rotaviruses on the genetic background of DS-1-like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G3P[8] strains.
新型 DS-1 样双基因群重组轮状病毒（具有马源样 G3 基因型的 DS-1 样 G3P[8]株）的出现和迅速传播最近已在多个国家报道。在 2015-2016 年日本轮状病毒监测期间，从患有严重腹泻的儿童中鉴定出了三株 DS-1 样 G3P[8]株。在本研究中，我们对这三株病毒进行了全基因组测序和特征分析。在全基因组分析中，所有三株病毒均显示出独特的基因型组合，包括基因群 1 和 2 基因：G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2。系统进化分析显示，三株病毒的 11 个基因均与日本 DS-1 样 G1P[8]和/或日本马源样 G3P[4]人源株密切相关。因此，这三株研究株被认为是重组株，它们在 DS-1 样 G1P[8]株的遗传背景上从马源 G3 轮状病毒获得了 G3-VP7 基因。我们的观察结果将为新型 DS-1 样 G3P[8]株的进化动态提供重要的见解。
