Department of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Faculty of Science Engineering & Technology, Swinburne University of Technology, Melbourne, VIC, 3122, Australia.
Angew Chem Int Ed Engl. 2018 Feb 23;57(9):2357-2361. doi: 10.1002/anie.201711441. Epub 2018 Jan 24.
Reversible immobilization of DNA and RNA is of great interest to researchers who seek to manipulate DNA or RNA in applications such as microarrays, DNA hydrogels, and gene therapeutics. However, there is no existing system that can rapidly capture and release intact nucleic acids. To meet this unmet need, we developed a functional hydrogel for rapid DNA/RNA capture and release based on the reversible photo-cycloaddition of psoralen and pyrimidines. The functional hydrogel can be easily fabricated through copolymerization of acrylamide with the synthesized allylated psoralen. The psoralen-functionalized hydrogel exhibits effective capture and release of nucleic acids spanning a wide range of lengths in a rapid fashion; over 90 % of the capture process is completed within 1 min, and circa 100 % of the release process is completed within 2 min. We observe no deleterious effects on the hybridization to the captured targets.
DNA 和 RNA 的可逆固定化引起了研究人员的极大兴趣，他们希望在微阵列、DNA 水凝胶和基因治疗等应用中操纵 DNA 或 RNA。然而，目前还没有能够快速捕获和释放完整核酸的现有系统。为了满足这一未满足的需求，我们开发了一种基于补骨脂素和嘧啶的可逆光环加成反应的快速 DNA/RNA 捕获和释放的功能水凝胶。该功能水凝胶可以通过丙烯酰胺与合成的烯丙基补骨脂素共聚轻松制备。该补骨脂素功能化水凝胶能够快速有效地捕获和释放广泛长度范围的核酸；超过 90%的捕获过程在 1 分钟内完成，大约 100%的释放过程在 2 分钟内完成。我们观察到对捕获靶标杂交没有有害影响。
