Department of International Agricultural Technology and Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.
Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
Virology. 2018 Mar;516:1-20. doi: 10.1016/j.virol.2018.01.001. Epub 2018 Jan 6.
The viral infection of plants may cause various physiological symptoms associated with the reprogramming of plant gene expression. However, the molecular mechanisms and associated genes underlying disease symptom development in plants infected with viruses are largely unknown. In this study, we employed RNA sequencing for in-depth molecular characterization of the transcriptional changes associated with the development of distinct symptoms induced by tomato chlorosis virus (ToCV) and tomato yellow leaf curl virus (TYLCV) in tomato. Comparative analysis of differentially expressed genes revealed that ToCV and TYLCV induced distinct transcriptional changes in tomato and resulted in the identification of important genes responsible for the development of symptoms of ToCV (i.e., chlorosis and anthocyanin accumulation) and TYLCV (i.e., yellowing, stunted growth, and leaf curl). Our comprehensive transcriptome analysis can provide molecular strategies to reduce the severity of disease symptoms as well as new insights for the development of virus-resistant crops.
植物的病毒感染可能导致与植物基因表达重编程相关的各种生理症状。然而，感染病毒的植物中与疾病症状发展相关的分子机制和相关基因在很大程度上尚不清楚。在这项研究中，我们采用 RNA 测序对番茄黄化曲叶病毒（TYLCV）和番茄褪绿病毒（ToCV）感染番茄后不同症状发展相关的转录变化进行了深入的分子特征分析。差异表达基因的比较分析表明，ToCV 和 TYLCV 在番茄中诱导了不同的转录变化，并鉴定出负责 ToCV（即褪绿和花青素积累）和 TYLCV（即黄化、生长迟缓和叶片卷曲）症状发展的重要基因。我们的综合转录组分析可以为减轻疾病症状的严重程度提供分子策略，并为抗病毒作物的发展提供新的见解。
