Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, PR China; Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610064, PR China.
J Colloid Interface Sci. 2018 Mar 15;514:733-739. doi: 10.1016/j.jcis.2018.01.014. Epub 2018 Jan 4.
Chemical reduction of graphene oxide (GO) is a simple and inexpensive method for the large-scale production of graphene-based materials. A suitable reducing agent, especially a green reductant, is in high demand for the production of reduced GO (RGO). Vancomycin, a glycopeptide antibiotic used to treat a variety of Gram-positive bacterial infections, was used to chemically reduce GO at a weak alkaline pH. As far as we know, this is the first report of reduction of GO by a glycopeptide antibiotic. The resulting vancomycin-decorated RGO (RGO-Van) was characterized by UV-visible adsorption and Raman spectroscopies, X-ray diffraction pattern and atomic force microscopy (AFM). The antibacterial effect of the RGO-Van suspension was investigated by the bacterial growth curves. The RGO-Van sheet can be fabricated into paper-like film through vacuum filtration. The antibacterial property of the as-obtained RGO-Van film was assessed by the inhibition zone test, and the bacterial adhesion assay. The antibacterial efficacy of the RGO-Van film was also verified by treatment of wound infection caused by Staphylococcus aureus (S. aureus) in a rat infection model.
氧化石墨烯（GO）的化学还原是大规模生产基于石墨烯的材料的一种简单且廉价的方法。对于还原氧化石墨烯（RGO）的生产，需要一种合适的还原剂，特别是一种绿色还原剂。万古霉素是一种糖肽类抗生素，用于治疗多种革兰氏阳性菌感染，它被用于在弱碱性 pH 下化学还原 GO。据我们所知，这是首次报道糖肽类抗生素还原 GO。通过紫外-可见吸收光谱和拉曼光谱、X 射线衍射图谱和原子力显微镜（AFM）对所得的万古霉素修饰的 RGO（RGO-Van）进行了表征。通过细菌生长曲线研究了 RGO-Van 悬浮液的抗菌效果。通过真空过滤可将 RGO-Van 片制成类似纸张的薄膜。通过抑菌圈试验和细菌黏附试验评估了所获得的 RGO-Van 薄膜的抗菌性能。通过金黄色葡萄球菌（S. aureus）感染大鼠模型治疗伤口感染，也验证了 RGO-Van 薄膜的抗菌功效。
