Danesh Hiva, Hashemi Mohammad, Bizhani Fatemeh, Hashemi Seyed Mehdi, Bahari Gholamreza
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Gene. 2018 Mar 20;647:73-78. doi: 10.1016/j.gene.2018.01.025. Epub 2018 Jan 6.
MicroRNAs (miRNAs) regulate genes expression by directly binding to the 3' untranslated region (3'UTR) of specific target mRNAs. Single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) are proposed to be important in the development of breast cancer (BC). In the present study, we conducted a case-control study with 266 BCE patients and 288 control women to examine the possible association of miRNAs polymorphisms (miR-100 rs1834306, miR-124-1 rs531564, miR-218-2 rs11134527, miR-301b rs384262, miR-605 rs2043556, and miR-4293 rs12220909) with BC susceptibility. Genotyping was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The findings showed miR-218-2 rs11134527 variant increased the risk of BC (OR = 7.70, 95%CI = 3.84-15.43, P < .0001, GA vs GG and OR = 6.86, 95%CI = 3.47-13.57, P < .0001, A vs G). Regarding miR-301b rs384262 polymorphism, we observed that this variant significantly increased the risk of BC (OR = 3.12, 95%CI = 2.20-4.45, P < .0001, AG vs AA and OR = 2.22, 95%CI = 1.68-2.93, P < .0001, G vs A). Our findings did not support an association between miR-100 rs1834306, miR-124-1 rs531564, miR-605 rs2043556 and miR-4293 rs12220909 polymorphism and the risk of BC. In conclusion, the finding showed that miR-218-2 rs11134527 and miR-301b rs384262 variant might contribute to increase the risk of BC in a sample of Iranian population. Additional studies with larger sample sizes and different ethnicities are necessary to confirm our finding.
微小RNA（miRNA）通过直接结合特定靶标mRNA的3'非翻译区（3'UTR）来调控基因表达。微小RNA（miRNA）中的单核苷酸多态性（SNP）被认为在乳腺癌（BC）的发生发展中具有重要作用。在本研究中，我们进行了一项病例对照研究，纳入266例乳腺癌患者和288例对照女性，以检测miRNA多态性（miR-100 rs1834306、miR-124-1 rs531564、miR-218-2 rs11134527、miR-301b rs384262、miR-605 rs2043556和miR-4293 rs12220909）与乳腺癌易感性之间的可能关联。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因分型。结果显示，miR-218-2 rs11134527变异增加了乳腺癌风险（OR = 7.70，95%CI = 3.84 - 15.43，P <.0001，GA与GG比较；OR = 6.86，95%CI = 3.47 - 13.57，P <.0001，A与G比较）。对于miR-301b rs384262多态性，我们观察到该变异显著增加了乳腺癌风险（OR = 3.12，95%CI = 2.20 - 4.45，P <.0001，AG与AA比较；OR = 2.22，95%CI = 1.68 - 2.93，P <.0001，G与A比较）。我们的研究结果不支持miR-100 rs1834306、miR-124-1 rs531564、miR-605 rs2043556和miR-4293 rs12220909多态性与乳腺癌风险之间存在关联。总之，研究结果表明，在伊朗人群样本中，miR-218-2 rs11134527和miR-301b rs384262变异可能会增加乳腺癌风险。需要进行更多样本量和不同种族的研究来证实我们的发现。
