Zhu Feng, Ou Qinfang, Zheng Jian
Dept. of Pulmonology, Wuxi No.5 People's Hospital Affiliated to Jiangnan University, Wuxi, China.
Iran J Public Health. 2018 Jan;47(1):18-23.
This paper aims to explore the application value of tuberculosis-specific enzyme-linked immunospot assay (T-SPOT.TB) in the diagnosis of tuberculosis.
Fifty one patients with tuberculosis (TB) admitted to Wuxi No.5 People's Hospital, Wuxi, China from June 2015 to June 2017 were selected as the TB group, and 40 patients without tuberculosis admitted in the same period were randomly selected as the non-TB group. Patients in the two groups received T-SPOT.TB, TB antibody (TB-Ab) test and mycobacterium TB deoxyribonucleic acid (TB-DNA) test, and the results were compared.
Comparisons of the sensitivity of the three methods showed that the sensitivity of T-SPOT.TB was the highest, followed by TB-DNA from sputum samples, and that of TB-Ab was the lowest. The specificity of TB-Ab was the highest, followed by T-SPOT.TB, and that of TB-DNA from sputum samples was the lowest. In the receiver operating characteristic (ROC) curve analysis, the area under curve (AUC) of T-SPOT.TB (0.896) was the highest, followed by TB-DNA from sputum samples (0.772), and that of sputum smears (0.698) was the lowest.
T-SPOT.TB can quickly and accurately determine the presence of tuberculosis infection, and it is a non-invasive examination, which can further assist in the diagnosis and guide the treatment.
本文旨在探讨结核特异性酶联免疫斑点试验（T-SPOT.TB）在结核病诊断中的应用价值。
选取2015年6月至2017年6月在中国无锡第五人民医院收治的51例结核病患者作为结核组，同期随机选取40例非结核病患者作为非结核组。两组患者均接受T-SPOT.TB、结核抗体（TB-Ab）检测及结核分枝杆菌脱氧核糖核酸（TB-DNA）检测，并比较结果。
三种方法敏感性比较显示，T-SPOT.TB敏感性最高，其次为痰标本TB-DNA，TB-Ab敏感性最低。TB-Ab特异性最高，其次为T-SPOT.TB，痰标本TB-DNA特异性最低。在受试者工作特征（ROC）曲线分析中，T-SPOT.TB曲线下面积（AUC）（0.896）最高，其次为痰标本TB-DNA（0.772），痰涂片（0.698）最低。
T-SPOT.TB能快速准确判断结核感染情况，是一种无创检查，可进一步辅助诊断并指导治疗。
