Department of Radiology, Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, Tianjin, 300052, China.
College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin, 300071, China.
Adv Mater. 2018 Feb;30(8). doi: 10.1002/adma.201704408. Epub 2018 Jan 10.
Current antigen-encapsulated multifunctional nanovaccines for oncotherapy suffer from limited antigen loading efficiency, low yield, tedious manufacture, and systemic toxicity. Here, an antigen-directed strategy for the fabrication of multifunctional nanovaccine with ultrahigh antigen loading efficiency in a facile way for tumor photothermal-immunotherapy is shown. As a proof of concept, a model antigen ovalbumin (OVA) is used as a natural carrier to load a representative theranostic agent indocyanine green (ICG). Mixing OVA and ICG in aqueous solution gives the simplest multifunctional nanovaccine so far. The nanovaccine owns antigen loading efficiency of 80.8%, high yield of >90%, intense near-infrared absorption and fluorescence, excellent reproducibility, good aqueous solubility and stability, and favorable biocompatibility. These merits not only guarantee sensitive labeling/tracking and efficient stimulation of dendritic cells, but also reliable imaging-guided photothermal-immunotherapy of tumors and tumor prevention. The proposed strategy provides a facile and robust method for large-scale and reproducible fabrication of multifunctional nanovaccines with ultrahigh antigen loading efficiency for tumor therapy.
目前的抗原包封多功能纳米疫苗在肿瘤治疗中存在抗原负载效率有限、产量低、制造繁琐和全身毒性等问题。在这里，展示了一种抗原导向的方法，用于制备具有超高抗原负载效率的多功能纳米疫苗，用于肿瘤光热免疫治疗，操作简单。作为概念验证，使用模型抗原卵清蛋白 (OVA) 作为天然载体来负载代表性治疗剂吲哚菁绿 (ICG)。将 OVA 和 ICG 在水溶液中混合得到迄今为止最简单的多功能纳米疫苗。该纳米疫苗的抗原负载效率为 80.8%，产率>90%，具有强烈的近红外吸收和荧光，良好的重现性，良好的水溶性和稳定性，以及良好的生物相容性。这些优点不仅保证了敏感的标记/跟踪和有效刺激树突状细胞，还保证了肿瘤的成像引导光热免疫治疗和肿瘤预防的可靠性。所提出的策略为制备具有超高抗原负载效率的多功能纳米疫苗提供了一种简单、稳健的方法，可用于肿瘤治疗。
