Stöhr L, Altmeyer P, Sessler M J, Scharrer I, Helm E B, Holzmann H
Z Hautkr. 1985 Aug 1;60(15):1214-23.
In order to reveal the activity of polymorphonuclear neutrophil leukocytes (PMNL) representing the first step of defence against infections, measurements of chemiluminescence (CL) were performed in patients suffering from acquired immune deficiency syndrome (AIDS), lymphadenopathy, or hemophilia. In comparison with healthy controls, AIDS patients revealed significant reduction (about 50 per cent) of phagocytic, i.e. CL activity of neutrophils, which had been induced by Zymosan. Only part of the patients suffering from lymphadenopathy answered with decreased granulocyte activity on the application of Zymosan. If concanavalin A was used as stimulant of metabolic activity of PMNL-independently of phagocytosis-again AIDS and some of the lymphadenopathy patients showed a markedly reduced neutrophil response. In conclusion it should be stated that there is some evidence for at least two defects of cellular immunity associated with AIDS and to some extent, with AIDS-endangered homosexuals suffering from lymphadenopathy: first the defect of PMNL to answer to concanavalin A with increased metabolic activity, and secondly the defect of PMNL to start phagocytosis induced by Zymosan with a subsequent release of oxygen radicals which are measurable as chemiluminescence. The appraisal of granulocyte activity by means of measurements of chemiluminescence might become an additional criterion for AIDS diagnostics.
为了揭示作为抗感染防御第一步的多形核中性粒细胞（PMNL）的活性，对获得性免疫缺陷综合征（AIDS）、淋巴结病或血友病患者进行了化学发光（CL）测量。与健康对照相比，AIDS患者的吞噬作用即中性粒细胞的CL活性显著降低（约50%），这是由酵母聚糖诱导的。只有部分淋巴结病患者在应用酵母聚糖后粒细胞活性降低。如果使用刀豆球蛋白A作为PMNL代谢活性的刺激物（与吞噬作用无关），AIDS患者和一些淋巴结病患者的中性粒细胞反应再次明显降低。总之，应该指出的是，有证据表明与AIDS相关以及在一定程度上与患有淋巴结病的AIDS高危同性恋者相关的细胞免疫至少存在两种缺陷：第一，PMNL对刀豆球蛋白A反应时代谢活性增加的缺陷；第二，PMNL启动由酵母聚糖诱导的吞噬作用并随后释放可作为化学发光测量的氧自由基的缺陷。通过化学发光测量评估粒细胞活性可能成为AIDS诊断的一个额外标准。
