Department of Biochemistry, Biophysics and General Pathology, University of Campania "Luigi Vanvitelli", Naples, Italy.
Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.
J Cell Physiol. 2018 Dec;233(12):9345-9353. doi: 10.1002/jcp.26427. Epub 2018 Jun 19.
This study was conceived to evaluate the effects of three different diets on body composition, metabolic parameters, and serum oxidative status. We enrolled three groups of healthy men (omnivores, vegetarians, and vegans) with similar age, weight and BMI, and we observed a significant decrease in muscle mass index and lean body mass in vegan compared to vegetarian and omnivore groups, and higher serum homocysteine levels in vegetarians and vegans compared to omnivores. We studied whether serum from omnivore, vegetarian, and vegan subjects affected oxidative stress, growth and differentiation of both cardiomyoblast cell line H9c2 and H-H9c2 (H9c2 treated with H O to induce oxidative damage). We demonstrated that vegan sera treatment of both H9c2 and H-H9c2 cells induced an increase of TBARS values and cell death and a decrease of free NO compared to vegetarian and omnivorous sera. Afterwards, we investigated the protective effects of vegan, vegetarian, and omnivore sera on the morphological changes induced by H O in H9c2 cell line. We showed that the omnivorous sera had major antioxidant and differentiation properties compared to vegetarian and vegan sera. Finally, we evaluated the influence of the three different groups of sera on MAPKs pathway and our data suggested that ERK expression increased in H-H9c2 cells treated with vegetarian and vegan sera and could promote cell death. The results obtained in this study demonstrated that restrictive vegan diet could not prevent the onset of metabolic and cardiovascular diseases nor protect by oxidative damage.
本研究旨在评估三种不同饮食对人体成分、代谢参数和血清氧化状态的影响。我们招募了三组年龄、体重和 BMI 相似的健康男性（杂食者、素食者和严格素食者），与素食者和杂食者相比，严格素食者的肌肉质量指数和瘦体重明显下降，素食者和严格素食者的血清同型半胱氨酸水平高于杂食者。我们研究了杂食者、素食者和严格素食者的血清是否会影响心肌细胞系 H9c2 和 H-H9c2（用 H O 处理 H9c2 以诱导氧化损伤）的氧化应激、生长和分化。我们证明，与素食者和杂食者的血清相比，严格素食者的血清处理 H9c2 和 H-H9c2 细胞会导致 TBARS 值升高、细胞死亡增加和游离 NO 减少。之后，我们研究了严格素食者、素食者和杂食者的血清对 H9c2 细胞系中由 H O 引起的形态变化的保护作用。我们表明，与素食者和严格素食者的血清相比，杂食者的血清具有更强的抗氧化和分化特性。最后，我们评估了三组血清对 MAPKs 通路的影响，我们的数据表明，在素食者和严格素食者的血清处理下，H-H9c2 细胞中 ERK 表达增加，可能促进细胞死亡。本研究的结果表明，严格的素食饮食既不能预防代谢和心血管疾病的发生，也不能防止氧化损伤。
