Stošić M, Matavulj M, Marković J
a Faculty of Technical Sciences, Department of Environmental engineering and Occupational Safety and Health , University of Novi Sad , Novi Sad , Serbia.
b Faculty of Sciences, Department of Biology and Ecology , University of Novi Sad , Novi Sad , Serbia.
Biotech Histochem. 2018;93(2):89-98. doi: 10.1080/10520295.2017.1393562. Epub 2018 Jan 10.
Acrylamide (AA) is a well-known industrial monomer with carcinogenic, mutagenic, neurotoxic and endocrine disruptive effects on living organisms. AA has been the subject of renewed interest owing to its presence in various food products. We investigated the potential adverse effects of oral AA treatment on the endocrine pancreas of juvenile rats using histochemical, immunohistochemical, stereological and biochemical methods. Thirty juvenile male Wistar rats were divided into one control and two AA treatment groups: one treated with 25 mg/kg AA and the other treated with 50 mg/kg AA for 21 days. We found a significant decrease in β-cell mass. The significant decrease in β-cell optical density and unchanged blood glucose levels indicate that normoglycemia in AA treated rats may result from intensive exocytosis of insulin-containing secretory granules. By contrast with β-cells, we observed increased α-cell mass. The slight increase in α-cell cytoplasmic volume suggests retention of glucagon in α-cells, which is consistent with the significant increase in α-cell optical density for AA treated animals. The number of islets of Langerhans did not change significantly in AA treated groups. Our findings suggest that AA treatment causes decreased β-cell mass and moderate α-cell mass increase in the islets of Langerhans of juvenile male Wistar rats.
丙烯酰胺（AA）是一种广为人知的工业单体，对生物具有致癌、致突变、神经毒性和内分泌干扰作用。由于其存在于各种食品中，AA再次引起了人们的关注。我们使用组织化学、免疫组织化学、体视学和生物化学方法，研究了口服AA对幼年大鼠内分泌胰腺的潜在不良影响。将30只幼年雄性Wistar大鼠分为一个对照组和两个AA治疗组：一组用25mg/kg AA处理，另一组用50mg/kg AA处理，持续21天。我们发现β细胞数量显著减少。β细胞光密度显著降低而血糖水平不变，这表明经AA处理的大鼠血糖正常可能是含胰岛素分泌颗粒大量胞吐的结果。与β细胞相反，我们观察到α细胞数量增加。α细胞胞质体积略有增加，表明胰高血糖素在α细胞中潴留，这与经AA处理动物的α细胞光密度显著增加一致。在经AA处理的组中，胰岛数量没有显著变化。我们的研究结果表明，AA处理会导致幼年雄性Wistar大鼠胰岛中β细胞数量减少，α细胞数量适度增加。
