Stošić Milena, Matavulj Milica, Marković Jelena
University of Novi Sad, Faculty of Technical Science, Department of Environmental engineering and Occupational Safety and Health, Trg Dositeja Obradovića 6, 21000 Novi Sad, Serbia.
University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia.
Acta Histochem. 2018 Apr;120(3):228-235. doi: 10.1016/j.acthis.2018.02.002. Epub 2018 Feb 15.
Acrylamide (AA) is a toxic substance, used to synthesize polymers for industrial and laboratory processes. Also, AA is a food contaminant formed during the high temperature preparation of carbohydrate-rich food. The main subject of this study was to examine effects of subchronic AA treatment on the islets of Langerhans of adult rats. Adult male Wistar rats were orally treated with 25 or 50 mg/kg bw of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of glucagon and insulin expression and stereological analyses of pancreatic alpha and beta cells were performed. Serum insulin and glucose levels were measured. Analysis of glucagon-immunostained sections revealed a dose-dependent increase of intensity of glucagon immunopositive signal, alpha cell surface and numerical densities, volume density of alpha cell nuclei and nucleocytoplasmic ratio in AA-treated groups compared to the control. In insulin-immunolabeled pancreatic sections in AA-treated animals was observed decrease of intensity of insulin immunopositive signal, beta cell surface, numerical and volume densities and volume density of beta cell cytoplasm. Serum insulin and glucose concentrations remained unchanged after both AA treatments. The number of islets of Langerhans was not affected by AA treatment. Our results suggest that AA subchronic treatment of adult rats leads to remodeling of islet of Langerhans characterized by alpha cell expansion and beta cell mass reduction.
丙烯酰胺（AA）是一种有毒物质，用于合成工业和实验室过程中的聚合物。此外，AA还是富含碳水化合物食物高温制备过程中形成的一种食品污染物。本研究的主要目的是检测亚慢性AA处理对成年大鼠胰岛的影响。成年雄性Wistar大鼠口服25或50mg/kg体重的AA，持续3周。对胰高血糖素和胰岛素表达进行定性和定量免疫组织化学评估，并对胰腺α细胞和β细胞进行体视学分析。检测血清胰岛素和葡萄糖水平。对胰高血糖素免疫染色切片的分析显示，与对照组相比，AA处理组中胰高血糖素免疫阳性信号强度、α细胞表面和数量密度、α细胞核体积密度和核质比呈剂量依赖性增加。在AA处理动物的胰岛素免疫标记胰腺切片中，观察到胰岛素免疫阳性信号强度、β细胞表面、数量和体积密度以及β细胞胞质体积密度降低。两种AA处理后血清胰岛素和葡萄糖浓度均保持不变。朗格汉斯胰岛的数量不受AA处理的影响。我们的结果表明，成年大鼠亚慢性AA处理导致朗格汉斯胰岛重塑，其特征为α细胞扩张和β细胞数量减少。
