Herrera-Calderon Oscar, Santiváñez-Acosta Rocio, Pari-Olarte Bertha, Enciso-Roca Edwin, Campos Montes Vicente Martin, Luis Arroyo Acevedo Jorge
Academic Department of Pharmaceutical Sciences, Faculty of Pharmacy and Biochemistry, Universidad Nacional San Luis Gonzaga de Ica, Ica, Peru.
National Center of Intercultural Health, National Institute of Health, Lima, Peru.
J Tradit Complement Med. 2017 Apr 20;8(1):95-99. doi: 10.1016/j.jtcme.2017.03.001. eCollection 2018 Jan.
(CA) rhizomes have demonstrated different properties on nervous system. However, the leaves still have not studied to treat epilepsy. The aim of this study was to determine the effect of CA ethanolic extract on pentylenetetrazol (PTZ) induced seizures in mice as well as measuring its antioxidant activity and . Mice were divided into five groups: (1) control (PTZ 80 mg/kg; i.p.), (2) PTZ-Diazepam (1 mg/kg; i.p.), (3-5) PTZ-CA 50, PTZ-CA 150 and PTZ-CA 300 (50, 150 and 300 mg/kg of CA extract, 30 min prior to each PTZ injection). The PTZ-CA 150 group showed lower seizure scores (P < 0.01), latency (P < 0.01), frequency (P < 0.01) and duration (P < 0.01) than control group. The antioxidant activity of CA extract scavenged DPPH radical showed IC 50 = 16.9 ± 0.1 μg/mL and TEAC = 2.28 ± 0.08, mmol trolox/g of extract, the content of gamma amino butyric acid (GABA) and malondialdehyde (MDA) were significantly high (P < 0.01) at dose of 150 mg/kg (82 ± 1.2 ng/g tissue; 1.0 ± 2.2 mol/g tissue, respectively). The present research demonstrated that CA extract possesses a potential effect to prevent PTZ induced seizures, antioxidant activity in addition to increase GABA levels.
（CA）根茎已显示出对神经系统的不同特性。然而，其叶子在治疗癫痫方面仍未得到研究。本研究的目的是确定CA乙醇提取物对戊四氮（PTZ）诱导的小鼠癫痫发作的影响，并测量其抗氧化活性。将小鼠分为五组：（1）对照组（PTZ 80mg/kg；腹腔注射），（2）PTZ-地西泮组（1mg/kg；腹腔注射），（3 - 5）PTZ-CA 50、PTZ-CA 150和PTZ-CA 300组（分别在每次PTZ注射前30分钟给予50、150和300mg/kg的CA提取物）。PTZ-CA 150组的癫痫发作评分（P < 0.01）、潜伏期（P < 0.01）、发作频率（P < 0.01）和持续时间（P < 0.01）均低于对照组。CA提取物清除DPPH自由基的抗氧化活性显示IC50 = 16.9 ± 0.1μg/mL，TEAC = 2.28 ± 0.08mmol Trolox/g提取物，在150mg/kg剂量下，γ-氨基丁酸（GABA）和丙二醛（MDA）的含量显著升高（P < 0.01）（分别为82 ± 1.2ng/g组织；1.0 ± 2.2mol/g组织）。本研究表明，CA提取物具有预防PTZ诱导的癫痫发作的潜在作用、抗氧化活性以及增加GABA水平的作用。
