鞣花酸对戊四氮诱导雄性小鼠惊厥的抗惊厥作用中 NMDA 受体的作用。
The Role of the NMDA Receptor in the Anticonvulsant Effect of Ellagic Acid in Pentylenetetrazole-Induced Seizures in Male Mice.
机构信息
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
出版信息
Behav Neurol. 2022 May 11;2022:9015842. doi: 10.1155/2022/9015842. eCollection 2022.
METHODS
In this experimental study, 64 mice were divided into 8 groups and received the following: normal saline; EA at doses of 6.25, 12.5, and 25 mg/kg; NMDA agonist at a dose of 75 mg/kg; NMDA antagonist (ketamine) at a dose of 0.5 mg/kg; an effective dose of EA plus NMDA agonist; and a subeffective dose of EA plus ketamine. We induced seizure using intravenous administration of PTZ. 60 minutes before induction of seizure, drugs were administrated. Duration lasts to seizure-induced was measured. Finally, the gene expression of NMDA receptor subunits ( and ) was assessed in the prefrontal cortex.
RESULTS
Results showed that EA increased the seizure threshold and decreased the expression of Nr2a and Nr2b. We determined that ketamine potentiated and NMDA attenuated the effects of subeffective and effective doses of EA.
CONCLUSION
EA probably via attenuation of the NMDA-R pathway possesses an anticonvulsant effect in PTZ-induced seizure in mice.
方法
在这项实验研究中，将 64 只小鼠分为 8 组，分别接受以下处理：生理盐水；EA 剂量分别为 6.25、12.5 和 25mg/kg；NMDA 激动剂 75mg/kg；NMDA 拮抗剂（氯胺酮）0.5mg/kg；EA 的有效剂量加 NMDA 激动剂；以及 EA 的亚有效剂量加氯胺酮。我们通过静脉注射 PTZ 诱导癫痫发作。在诱导癫痫发作前 60 分钟给予药物。测量从诱导癫痫发作到发作持续的时间。最后，评估前额叶皮层中 NMDA 受体亚基（和）的基因表达。
结果
结果表明，EA 增加了癫痫发作的阈值，并降低了 Nr2a 和 Nr2b 的表达。我们确定，氯胺酮增强了亚有效剂量和有效剂量 EA 的作用，而 NMDA 则减弱了 EA 的作用。
结论
EA 可能通过抑制 NMDA-R 通路，在 PTZ 诱导的小鼠癫痫发作中具有抗惊厥作用。
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