Goldman Ellen R, Broussard Aeris, Anderson George P, Liu Jinny L
US Naval Research Laboratory, Center for Biomolecular Science and Engineering, 4555 Overlook Ave SW, Washington DC 20375, USA.
US Naval Research Laboratory, Historically Black Colleges and Universities/Minority Institutions Summer Internship Program, 4555 Overlook Ave SW, Washington, DC 20375, USA.
Heliyon. 2017 Dec 28;3(12):e00474. doi: 10.1016/j.heliyon.2017.e00474. eCollection 2017 Dec.
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications. Constructs in which several single domain antibodies are genetically fused serially, as well as those in which single domain antibodies are genetically linked with domains that naturally form multimers, yield improvement in apparent binding affinity due to avidity. Here, using a single domain antibody that binds envelope protein from the Dengue virus, we demonstrated the construction of single domain antibody dimers using the Bglbrick cloning strategy. Constructing single domain antibodies and multimerization domains as Bglbrick parts enables the easy mixing and matching of parts. The dimeric constructs provided enhanced fluorescent signal in assays for detection of Dengue virus like particles over the monomeric single domain antibody.
单域抗体是源自骆驼科动物重链抗体的重组表达可变结构域，常用于检测和治疗应用，通常以多聚体形式表达。将多个单域抗体进行基因串联融合构建的结构，以及将单域抗体与天然形成多聚体的结构域进行基因连接构建的结构，由于亲和力作用，其表观结合亲和力会有所提高。在此，我们使用一种能结合登革病毒包膜蛋白的单域抗体，通过Bglbrick克隆策略展示了单域抗体二聚体的构建。将单域抗体和多聚化结构域构建为Bglbrick组件，能够方便地对组件进行混合和匹配。在检测登革病毒样颗粒的实验中，二聚体构建体比单体单域抗体提供了更强的荧光信号。
