Department of Prosthetic Dental Science, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Dental University Hospital, King Saud University, Riyadh, Saudi Arabia.
J Prosthodont. 2019 Apr;28(4):e896-e901. doi: 10.1111/jopr.12744. Epub 2018 Jan 11.
To evaluate the cytotoxic effects of different hemostatic agents (including Expasyl) on human gingival fibroblasts (HGFs) in vitro.
HGFs were cultured and exposed to either no medicament treatment or 1:200 dilution of six different hemostatic agents (Hemox-A, Hemodent, Astringedent, Vicostat, Expasyl, 3M ESPE) for 2, 5, 10 minutes, 1 hour, and 24 hours. Toxicity to HGFs was determined by lactate dehydrogenase activity (LDH) and colorimetric (WST-1) assays. Two-tailed t-test was used for statistical analyses with α level set at 0.05.
The group-by-time interactions were significant for the LDH and WST-1 assays (p < 0.001). Evaluation of the cytotoxic effect of different hemostatic agents at different incubation time intervals on the cell membrane damage revealed that Astringedent showed the highest cytotoxic effect on HGFs compared to other agents with regards to untreated negative control cells at all incubation time intervals (p < 0.001). On the other hand, Expasyl showed the least cytotoxic effect with significant differences at 5 minutes and 1 hour (p < 0.001) in comparison to other agents.
LDH and WST-1 assays of hemostatic agents showed significant cytotoxic effect on HGFs at different time intervals. The data suggest that the risk for permanent tissue damage might be less significant when Expasyl is used during final impression procedure compared to when Astringedent is used.
评估不同止血剂（包括 Expasyl）对体外人牙龈成纤维细胞（HGFs）的细胞毒性作用。
培养 HGFs 并使其暴露于无药物处理或 6 种不同止血剂（Hemox-A、Hemodent、Astringedent、Vicostat、Expasyl、3M ESPE）1:200 稀释液中 2、5、10 分钟、1 小时和 24 小时。通过乳酸脱氢酶活性（LDH）和比色（WST-1）测定法来确定对 HGFs 的毒性。使用双尾 t 检验进行统计分析，α 值设定为 0.05。
LDH 和 WST-1 测定的组间时间相互作用均具有统计学意义（p<0.001）。评估不同止血剂在不同孵育时间间隔对细胞膜损伤的细胞毒性作用的结果表明，与未处理的阴性对照细胞相比，Astringedent 在所有孵育时间间隔内对 HGFs 的细胞毒性作用最高（p<0.001）。另一方面，与其他试剂相比，Expasyl 在 5 分钟和 1 小时时的细胞毒性作用最小，差异具有统计学意义（p<0.001）。
不同时间间隔的止血剂 LDH 和 WST-1 测定均对 HGFs 表现出显著的细胞毒性作用。数据表明，与使用 Astringedent 相比，在最终印模制取过程中使用 Expasyl 可能会降低对组织造成永久性损伤的风险。
