a Department of Fashion and Beauty Sciences , Osaka Shoin Women's University , Higashiosaka-shi , Osaka , Japan.
b Division in Beauty and Fashion Studies, Graduate School of Human Sciences , Osaka Shoin Women's University , Higashiosaka-shi , Osaka , Japan.
Pharm Dev Technol. 2019 Jan;24(1):99-104. doi: 10.1080/10837450.2018.1425885. Epub 2018 Jan 23.
Anionic surfactants are often used for cleaning and pharmaceutical purposes because of their strong surfactancy and foaming property. However, they are rarely ingested orally, the skin is a part of the human body most affected by surfactants. Barrier function of the skin is very strong, but the anionic surfactants can cause serious damages to it. Recently, amino acid-based surfactants have attracted attention as a safer option owing to their biocompatibility. Cytotoxicity examinations revealed that the amino acid-based surfactants are superior to sulfate-based surfactants. However, a systematical and comprehensive study related to the effect of these surfactants on skin barrier function has not yet been reported. In this work, skin permeation test using the skin of hairless mice and HPLC method is carried out. The material transmission speed through skin in a steady state was different between each surfactant treatment. We performed a comprehensive analysis of the effect of surfactants on skin barrier function and defined Transmission Index as an index for the degree of effect of surfactants. Glutamate series amino acid-based surfactant were effective to Transmission Index and we guessed the cause was due to adsorption. Based on the finding this study, we suggest using adsorptive property as a measure to the effect on the skin barrier function.
阴离子表面活性剂因其较强的表面活性和起泡性，常用于清洁和制药用途。然而，它们很少被口服摄入，皮肤是受表面活性剂影响最大的人体部位之一。皮肤的屏障功能非常强，但阴离子表面活性剂会对其造成严重损害。最近，由于氨基酸表面活性剂具有生物相容性，因此作为一种更安全的选择引起了人们的关注。细胞毒性试验表明，氨基酸表面活性剂优于硫酸盐表面活性剂。然而，关于这些表面活性剂对皮肤屏障功能影响的系统和全面的研究尚未见报道。在这项工作中，使用无毛小鼠的皮肤进行了皮肤渗透试验和 HPLC 法。在每种表面活性剂处理下，通过皮肤的物质传输速度在稳定状态下是不同的。我们对表面活性剂对皮肤屏障功能的影响进行了综合分析，并将传输指数定义为表面活性剂影响程度的指标。谷氨酸系列氨基酸表面活性剂对传输指数有效，我们猜测原因是由于吸附。基于这项研究的发现，我们建议使用吸附性作为衡量对皮肤屏障功能影响的指标。
