Bessaire Thomas, Mujahid Claudia, Beck Andrea, Tarres Adrienne, Savoy Marie-Claude, Woo Pei-Mun, Mottier Pascal, Desmarchelier Aurélien
a Nestlé Research Center, Institute of Food Safety and Analytical Science , Lausanne , Switzerland.
b Nestlé Quality Assurance Center, Singapore , Singapore.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2018 Apr;35(4):661-673. doi: 10.1080/19440049.2018.1426891. Epub 2018 Jan 22.
A fast and robust high performance LC-MS/MS screening method was developed for the analysis of β-lactam antibiotics in foods of animal origin: eggs, raw milk, processed dairy ingredients, infant formula, and meat- and fish-based products including baby foods. QuEChERS extraction with some adaptations enabled 23 drugs to be simultaneously monitored. Screening target concentrations were set at levels adequate to ensure compliance with current European, Chinese, US and Canadian regulations. The method was fully validated according to the European Community Reference Laboratories Residues Guidelines using 93 food samples of different composition. False-negative and false-positive rates were below 5% for all analytes. The method is adequate for use in high-routine laboratories. A 1-year study was additionally conducted to assess the stability of the 23 analytes in the working standard solution.
开发了一种快速、稳健的高效液相色谱-串联质谱（LC-MS/MS）筛选方法，用于分析动物源性食品中的β-内酰胺类抗生素，这些食品包括鸡蛋、生鲜乳、加工乳制品原料、婴儿配方奶粉以及基于肉类和鱼类的产品（包括婴儿食品）。经过一些改进的QuEChERS萃取法能够同时监测23种药物。筛选目标浓度设定在足以确保符合现行欧洲、中国、美国和加拿大法规的水平。该方法根据欧洲共同体参考实验室残留指南，使用93种不同成分的食品样本进行了全面验证。所有分析物的假阴性率和假阳性率均低于5%。该方法适用于高日常工作量的实验室。此外，还进行了一项为期1年的研究，以评估工作标准溶液中23种分析物的稳定性。
