Jiménez-López Domingo, Aguilar-Henonin Laura, González-Prieto Juan Manuel, Aguilar-Hernández Victor, Guzmán Plinio
Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Guanajuato, México.
Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, México.
PLoS One. 2018 Jan 11;13(1):e0190969. doi: 10.1371/journal.pone.0190969. eCollection 2018.
RING ubiquitin E3 ligases enclose a RING domain for ubiquitin ligase activity and associated domains and/or conserved motifs outside the RING domain that collectively facilitate their classification and usually reveal some of key information related to mechanism of action. Here we describe a new family of E3 ligases that encodes a RING-H2 domain related in sequence to the ATL and BTL RING-H2 domains. This family, named CTL, encodes a motif designed as YEELL that expands 21 amino acids next to the RING-H2 domain that is present across most eukaryotic lineages. E3 ubiquitin ligase BIG BROTHER is a plant CTL that regulates organ size, and SUMO-targeted ubiquitin E3 ligase RNF111/ARKADIA is a vertebrate CTL. Basal animal and vertebrate, as well as fungi species, encode a single CTL gene that constraints the number of paralogs observed in vertebrates. Conversely, as previously described in ATL and BTL families in plants, CTL genes range from a single copy in green algae and 3 to 5 copies in basal species to 9 to 35 copies in angiosperms. Our analysis describes key structural features of a novel family of E3 ubiquitin ligases as an integral component of the set of core eukaryotic genes.
环状泛素E3连接酶包含一个用于泛素连接酶活性的环状结构域以及环状结构域之外的相关结构域和/或保守基序，这些结构域和基序共同促进了它们的分类，并且通常揭示了一些与作用机制相关的关键信息。在这里，我们描述了一个新的E3连接酶家族，其编码一个与ATL和BTL的环状H2结构域序列相关的环状H2结构域。这个家族被命名为CTL，编码一个被设计为YEELL的基序，该基序在环状H2结构域旁边延伸21个氨基酸，存在于大多数真核生物谱系中。E3泛素连接酶“大哥哥”是一种调节器官大小的植物CTL，而SUMO靶向泛素E3连接酶RNF111/阿卡迪亚是一种脊椎动物CTL。基础动物和脊椎动物以及真菌物种都编码一个单一的CTL基因，这限制了在脊椎动物中观察到的旁系同源基因的数量。相反，正如之前在植物的ATL和BTL家族中所描述的那样，CTL基因的数量范围从绿藻中的单拷贝到基础物种中的3至5个拷贝，再到被子植物中的9至35个拷贝。我们的分析描述了一个新型E3泛素连接酶家族的关键结构特征，它是核心真核基因集的一个组成部分。
