Center for Biologics Evaluation and Research, Division of Bacterial, Parasitic and Allergenic Products, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993-0002, USA.
Emory Comprehensive Glycomics Core, Emory University School of Medicine, Room 105H, Whitehead Biomedical Res. Bldg., 615 Michael Street, Atlanta, GA 30322, USA.
Glycobiology. 2018 Apr 1;28(4):223-232. doi: 10.1093/glycob/cwy002.
Here we present a Caenorhabditis elegans N-glycan shotgun array. This nematode serves as a model organism for many areas of biology including but not limited to tissue development, host-pathogen interactions, innate immunity, and genetics. Caenorhabditis elegans N-glycans contain structural motifs that are also found in other nematodes as well as trematodes and lepidopteran species. Glycan binding toxins that interact with C. elegans glycoconjugates also do so with some agriculturally relevant species, such as Haemonchus contortus, Ascaris suum, Oesophagostomum dentatum and Trichoplusia ni. This situation implies that protein-carbohydrate interactions seen with C. elegans glycans may also occur in other species with related glycan structures. Therefore, this array may be useful to study these relationships in other nematodes as well as trematode and insect species. The array contains 134 distinct glycomers spanning a wide range of C. elegans N-glycans including the subclasses high mannose, pauci mannose, high fucose, mammalian-like complex and phosphorylcholine substituted forms. The glycans presented on the array have been characterized by two-dimensional separation, ion trap mass spectrometry, and lectin affinity. High fucose glycans were well represented and contain many novel core structures found in C. elegans as well as other species. This array should serve as an investigative platform for carbohydrate binding proteins that interact with N-glycans of C. elegans and over a range of organisms that contain glycan motifs conserved with this nematode.
这里我们呈现了一个秀丽隐杆线虫 N-糖组 shotgun 阵列。这种线虫作为许多生物学领域的模式生物，包括但不限于组织发育、宿主-病原体相互作用、先天免疫和遗传学。秀丽隐杆线虫 N-聚糖含有结构基序，这些基序也存在于其他线虫以及吸虫和鳞翅目物种中。与秀丽隐杆线虫糖缀合物相互作用的糖结合毒素也与一些农业相关的物种相互作用，如旋毛虫、猪蛔虫、食道口线虫和烟草天蛾。这种情况意味着与秀丽隐杆线虫糖相互作用的蛋白-碳水化合物相互作用也可能发生在具有相关聚糖结构的其他物种中。因此，该阵列可能有助于研究其他线虫以及吸虫和昆虫物种中的这些关系。该阵列包含 134 种不同的糖，涵盖了广泛的秀丽隐杆线虫 N-聚糖，包括高甘露糖、少甘露糖、高岩藻糖、哺乳动物样复杂和磷酸胆碱取代形式。阵列上呈现的聚糖已经通过二维分离、离子阱质谱和凝集素亲和力进行了表征。高岩藻糖聚糖得到了很好的代表，并且包含许多在秀丽隐杆线虫以及其他物种中发现的新型核心结构。该阵列应该作为一个研究平台，用于研究与秀丽隐杆线虫 N-聚糖相互作用的碳水化合物结合蛋白，以及包含与这种线虫保守聚糖基序的一系列生物体。
