Sharma Akhilesh, Subramaniam Priya, Moiden Shebin
Department of Pedodontics and Preventive Dentistry, The Oxford Dental College and Hospital, Bengaluru, Karnataka, India.
Contemp Clin Dent. 2017 Oct-Dec;8(4):526-530. doi: 10.4103/ccd.ccd_103_17.
This study aimed to evaluate the levels of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children with early childhood caries.
Thirty children aged 36-60 months, with a deft score ≥5, were selected for the study. Before dental treatment, paraffin-stimulated whole saliva was collected in a sterile graduated cup for a period of 5 min. The saliva samples were quantitatively analyzed for levels of IgA, amylase, lactoferrin, and lysozyme using enzyme-linked immunosorbent assay. Comprehensive dental treatment was carried out in all the children including caries preventive procedures. A second sample of saliva was collected at 3 months following completion of dental treatment. Data obtained was subjected to statistical analysis using Student's -test.
The mean levels of salivary IgA was significantly reduced from 59.60 μg/ml to 56.42 μg/ml after dental treatment ( < 0.05). There was a significant reduction in the levels of salivary amylase from 115.78 μg/ml to 113.33 μg/ml ( < 0.001). Following dental treatment, salivary lactoferrin and lysozyme levels were significantly reduced from 3.76 μg/ml and 10.62 μg/ml to 3.44 μg/ml and 10.27 μg/ml, respectively ( < 0.001).
Levels of salivary IgA, amylase, lactoferrin, and lysozyme were reduced significantly at 3 months following comprehensive dental treatment.
本研究旨在评估幼儿龋齿患儿在综合牙科治疗前后唾液中免疫球蛋白A（IgA）、淀粉酶、乳铁蛋白和溶菌酶的水平。
选取30名年龄在36 - 60个月、乳牙龋失补牙指数（deft）评分≥5的儿童进行研究。在牙科治疗前，用无菌刻度杯收集石蜡刺激后的全唾液5分钟。采用酶联免疫吸附测定法对唾液样本中的IgA、淀粉酶、乳铁蛋白和溶菌酶水平进行定量分析。对所有儿童进行包括龋齿预防程序在内的综合牙科治疗。在牙科治疗完成后3个月收集第二份唾液样本。所获数据采用t检验进行统计分析。
牙科治疗后，唾液IgA的平均水平从59.60μg/ml显著降至56.42μg/ml（P<0.05）。唾液淀粉酶水平从115.78μg/ml显著降至113.33μg/ml（P<0.001）。牙科治疗后，唾液乳铁蛋白和溶菌酶水平分别从3.76μg/ml和10.62μg/ml显著降至3.44μg/ml和10.27μg/ml（P<0.001）。
综合牙科治疗后3个月，唾液IgA、淀粉酶、乳铁蛋白和溶菌酶水平显著降低。
