van der Mee-Marquet Nathalie L, Bénéjat Lucie, Diene Seydina M, Lemaignen Adrien, Gaïa Nadia, Smet Annemieke, Haesebrouck Freddy, Cherkaoui Abdessalam, Ducournau Astrid, Lacomme Sabrina, Gontier Etienne, Bernard Louis, Mégraud Francis, Goudeau Alain, Lehours Philippe, François Patrice
Service de Bactériologie, Virologie et Hygiène, Hôpital Trousseau, Réseau des Hygiénistes du Centre, CPIAS Centre Val de Loire, Centre Hospitalier Régional Universitaire, and UMR 1282 Infectiologie Santé Publique, Université François-Rabelais, Tours, France.
Laboratoire de Bactériologie, Centre National de Référence des Campylobacters et des Hélicobacters, Bordeaux, France.
Front Microbiol. 2017 Dec 18;8:2533. doi: 10.3389/fmicb.2017.02533. eCollection 2017.
We isolated from aerobic and anaerobic blood culture bottles from a febrile patient, a -like Gram negative, rod-shaped bacterium that MALDI-TOF MS failed to identify. Blood agar cultures incubated in a microaerobic atmosphere revealed a motile Gram negative rod, which was oxidase, catalase, nitrate reductase, esterase, and alkaline phosphatase positive. It grew at 42°C with no detectable urease activity. Antimicrobial susceptibility testing showed that the organism was susceptible to beta-lactams, gentamicin, erythromycin, and tetracycline but resistant to ciprofloxacin. Electronic microscopy analysis revealed a 3 × 0.5 μm curved rod bacterium harboring two sheathed amphitrichous flagella. Whole genome sequencing revealed a genome 1,708,265 base-pairs long with a GC content of 37.80% and a total of 1,697 coding sequences. The genomic analyses using the nucleotide sequences of the 16S rRNA gene, and genes, as well as the GyrA protein sequence, and the results of Average Nucleotide Identity and DNA-DNA hybridization suggest evidence for a novel species close to and belonging to the group of enterohepatic species. As soon as the particular peptide mass fingerprint of this pathogen is added to the spectral databases, MALDI-TOF MS technology will improve its identification from clinical specimens, especially in case of "sterile infection". We propose to associate the present strain with the Latin name of the place of isolation; Caesarodunum (Tours, France) and suggest "" for further description of this new bacterium.
我们从一名发热患者的需氧和厌氧血培养瓶中分离出一种革兰氏阴性、杆状细菌，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）未能对其进行鉴定。在微需氧环境中培养的血琼脂培养物显示出一种运动性革兰氏阴性杆菌，该菌氧化酶、过氧化氢酶、硝酸盐还原酶、酯酶和碱性磷酸酶呈阳性。它在42°C下生长，未检测到脲酶活性。抗菌药敏试验表明，该菌对β-内酰胺类、庆大霉素、红霉素和四环素敏感，但对环丙沙星耐药。电子显微镜分析显示，该菌为3×0.5μm的弯曲杆菌，带有两根鞘膜双端鞭毛。全基因组测序显示，其基因组长度为1,708,265个碱基对，GC含量为37.80%，共有1,697个编码序列。使用16S rRNA基因、基因以及GyrA蛋白序列的核苷酸序列进行的基因组分析，以及平均核苷酸同一性和DNA-DNA杂交结果表明，存在一种新的物种，与肠肝菌属物种接近且属于该组。一旦将这种病原体的特定肽质量指纹图谱添加到光谱数据库中，MALDI-TOF MS技术将改善其从临床标本中的鉴定，尤其是在“无菌感染”的情况下。我们建议将本菌株与分离地点的拉丁名称相关联；Caesarodunum（法国图尔），并建议使用“”对这种新细菌进行进一步描述。
