Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan, 31116, Republic of Korea.
R&D Center, Seoul Dairy Cooperative, Ansan, 15407, Republic of Korea.
Appl Microbiol Biotechnol. 2020 Jul;104(14):6273-6285. doi: 10.1007/s00253-020-10634-8. Epub 2020 May 11.
High-fat diet (HFD)-induced obesity has been associated with alteration of gut microbiota alongside body weight gain. In this study, the synbiotic effect of Lactobacillus gasseri 505 (LG) and Cudrania tricuspidata (CT) in HFD-induced mice was revealed. After feeding mice with high-fat diet for 10 weeks, combination of LG and CT (LG_CT) exhibited the greatest reduction in the final body weight (11.9%). Moreover, microbial diversity significantly increased, and Principal Coordinate Analysis (PCoA) revealed that the LG_CT group showed closer cluster to NORM. At phylum level, the Firmicutes/Bacteroidetes (F/B) ratio increased in HFD, and the abundance of Bacteroidetes was restored by LG and CT. At genus level, notable changes in Alistipes, Desulfovibrio, Bilophila, and Acetatifactor were observed. Helicobacter elevated to 16.2% in HFD and diminished dramatically to less than 0.01% in LG and/or CT. At species level, L. gasseri increased after the administration of LG (0.54%) and LG_CT (1.14%), suggesting that LG may grow and colonize in the gut and CT can function as a prebiotic. Finally, functional analysis revealed certain metabolic factors correlated with body weight and gut microbiota. This study serves as a potential basis for the application of L. gasseri 505 and C. tricuspidata in the prevention and treatment of diet-induced obesity.Key Points • Combination of L. gasseri (LG) and C. tricuspidata (CT) reduced body weight gain.• Microbial diversity significantly increased in LG_CT treatment.• Abundance of microorganisms involved with leanness increased in LG, CT, and LG_CT.• Body weight is associated with some metabolic functions of gut microbiota.
高脂肪饮食（HFD）诱导的肥胖与体重增加的同时肠道微生物群的改变有关。在这项研究中，揭示了植物乳杆菌 505（LG）和枳椇子（CT）在 HFD 诱导的小鼠中的共生效应。用高脂肪饮食喂养小鼠 10 周后，LG 和 CT 的组合（LG_CT）表现出最终体重（11.9%）的最大降低。此外，微生物多样性显著增加，主坐标分析（PCoA）显示 LG_CT 组与 NORM 更接近。在门水平上，厚壁菌门/拟杆菌门（F/B）比值在 HFD 中增加，LG 和 CT 恢复了拟杆菌门的丰度。在属水平上，观察到 Alistipes、Desulfovibrio、Bilophila 和 Acetatifactor 的显著变化。在 HFD 中，幽门螺旋杆菌升高到 16.2%，在 LG 和/或 CT 中急剧减少到低于 0.01%。在种水平上，给予 LG 后（0.54%）和 LG_CT（1.14%）后，L. gasseri 增加，表明 LG 可能在肠道中生长和定植，而 CT 可以作为一种益生元。最后，功能分析显示与体重和肠道微生物群相关的某些代谢因素。本研究为植物乳杆菌 505 和枳椇子在预防和治疗饮食诱导性肥胖中的应用提供了潜在依据。
LG 和 CT 的组合降低了体重增加。
LG_CT 治疗后微生物多样性显著增加。
LG、CT 和 LG_CT 中与苗条相关的微生物丰度增加。
体重与肠道微生物群的一些代谢功能有关。
