Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende, 87036, Rende, Italy.
Department of Biology, Ecology and Earth Sciences, University of Calabria, Arcavacata di Rende, 87036, Rende, Italy.
Eur J Nutr. 2019 Apr;58(3):965-979. doi: 10.1007/s00394-018-1609-1. Epub 2018 Jan 11.
Obesity is the main feature of a complex illness known as metabolic syndrome. Anti-obesogenic therapies are often associated with side effects and represent a high cost in conventional pharmacological approaches. New strategies based on natural remedies are under continuous investigation. Leopoldia comosa (L.) Parl. (L. comosa) is a spontaneous plant with diuretic, anti-inflammatory and antioxidant properties. Recently, a hypoglycemic activity mediated by inhibition of carbohydrate digestion has been identified. The aim of this study was to evaluate the effects of a diet supplemented with L. comosa extracts on a rat model of diet-induced obesity.
Leopoldia comosa bulb extracts were obtained using a dynamic extractor. Phytochemical properties and in vitro determination of the antioxidant activity and of the inhibitory effects on lipase and pancreatic amylase were performed. Rats were fed (12 weeks) a standard diet, or a high-fat diet (HFD), or an HFD plus L. comosa (20 or 60 mg/die) extracts. The metabolic and anthropometric parameters were recorded.
Results indicated that L. comosa inhibited lipase and pancreatic amylase activities. In vivo data showed that the supplementation with both doses of L. comosa extracts counteracted the HFD-dependent effects. It reduced body weight, abdominal obesity and dyslipidemia, and improved glucose tolerance with a reduction of lipidic tissue hypertrophy and liver steatosis, as compared to HFD-fed rat. In liver, L. comosa reduced protein expression levels of PEPCK and G6Pase.
We suggest that L. comosa extracts prevent obesity-dependent metabolic disorders. This paves the way for their therapeutic application as a natural anti-obesity drug.
肥胖是一种称为代谢综合征的复杂疾病的主要特征。抗肥胖疗法通常与副作用相关，并代表传统药物方法的高成本。基于天然药物的新策略正在不断研究中。Leopoldia comosa（L.）Parl.（L. comosa）是一种具有利尿、抗炎和抗氧化特性的野生植物。最近，发现了一种通过抑制碳水化合物消化介导的降血糖活性。本研究旨在评估用 L. comosa 提取物补充饮食对饮食诱导肥胖大鼠模型的影响。
使用动态提取器获得 Leopoldia comosa 鳞茎提取物。进行植物化学特性和体外抗氧化活性以及对脂肪酶和胰淀粉酶抑制作用的测定。大鼠喂食（12 周）标准饮食、高脂肪饮食（HFD）或 HFD 加 L. comosa（20 或 60mg/die）提取物。记录代谢和人体测量参数。
结果表明，L. comosa 抑制脂肪酶和胰淀粉酶活性。体内数据表明，两种剂量的 L. comosa 提取物的补充可拮抗 HFD 依赖性作用。与 HFD 喂养的大鼠相比，它降低了体重、腹部肥胖和血脂异常，并改善了葡萄糖耐量，减少了脂质组织肥大和肝脂肪变性。在肝脏中，L. comosa 降低了 PEPCK 和 G6Pase 的蛋白表达水平。
我们认为 L. comosa 提取物可预防肥胖相关的代谢紊乱。这为将其作为天然减肥药进行治疗应用铺平了道路。
