Tissue Engineering Research Group, Dept. of Anatomy, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland.
Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin (TCD), Dublin 2, Ireland.
J Tissue Eng Regen Med. 2018 Apr;12(4):e2147-e2150. doi: 10.1002/term.2638. Epub 2018 Mar 8.
Mimicking endochondral ossification to engineer constructs offers a novel solution to overcoming the problems associated with poor vascularisation in bone repair. This can be achieved by harnessing the angiogenic potency of hypertrophic cartilage. In this study, we demonstrate that tissue-engineered hypertrophically primed cartilage constructs can be developed from collagen-based scaffolds cultured with mesenchymal stem cells. These constructs were subsequently implanted into femoral defects in rats. It was evident that the constructs could support enhanced early stage healing at 4 weeks of these weight-bearing femoral bone defects compared to untreated defects. This study demonstrates the value of combining knowledge of development biology and tissue engineering in a developmental engineering inspired approach to tissue repair.
模拟软骨内骨化来设计构建物为克服骨修复中血管生成不良相关问题提供了一种新的解决方案。这可以通过利用肥大软骨的血管生成潜力来实现。在这项研究中，我们证明了可以从含有间充质干细胞的基于胶原蛋白的支架培养出工程化的肥大预刺激软骨构建物。随后将这些构建物植入大鼠的股骨缺损中。显然，与未处理的缺损相比，这些承重股骨骨缺损在 4 周时，构建物能够支持增强的早期愈合。这项研究证明了将发育生物学和组织工程学的知识结合起来，以发育工程启发的方法进行组织修复的价值。
