Cabriel Clément, Bourg Nicolas, Dupuis Guillaume, Lévêque-Fort Sandrine
Opt Lett. 2018 Jan 15;43(2):174-177. doi: 10.1364/OL.43.000174.
We propose a straightforward sample-based technique to calibrate the axial detection in 3D single-molecule localization microscopy. Using microspheres coated with fluorescent molecules, the calibration curves of point spread function-shaping or intensity-based measurements can be obtained over the imaging depth range. This experimental method takes into account the effect of the spherical aberration without requiring computational correction. We demonstrate its efficiency for astigmatic imaging in a 1.2 μm range above the coverslip.
我们提出了一种简单的基于样本的技术，用于校准三维单分子定位显微镜中的轴向检测。使用涂有荧光分子的微球，可以在成像深度范围内获得点扩散函数整形或基于强度测量的校准曲线。这种实验方法考虑了球差的影响，而无需进行计算校正。我们展示了其在盖玻片上方1.2μm范围内用于像散成像的效率。
