Wang Jingyu, Zhang Yongdeng
Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UK.
School of Life Sciences, Westlake University, Hangzhou 310024, China.
Biophys Rep. 2021 Aug 31;7(4):267-279. doi: 10.52601/bpr.2021.210015.
Fluorescence microscopy has become a routine tool in biology for interrogating life activities with minimal perturbation. While the resolution of fluorescence microscopy is in theory governed only by the diffraction of light, the resolution obtainable in practice is also constrained by the presence of optical aberrations. The past two decades have witnessed the advent of super-resolution microscopy that overcomes the diffraction barrier, enabling numerous biological investigations at the nanoscale. Adaptive optics, a technique borrowed from astronomical imaging, has been applied to correct for optical aberrations in essentially every microscopy modality, especially in super-resolution microscopy in the last decade, to restore optimal image quality and resolution. In this review, we briefly introduce the fundamental concepts of adaptive optics and the operating principles of the major super-resolution imaging techniques. We highlight some recent implementations and advances in adaptive optics for active and dynamic aberration correction in super-resolution microscopy.
荧光显微镜已成为生物学领域的一种常规工具，用于在最小程度干扰的情况下探究生命活动。虽然荧光显微镜的分辨率理论上仅受光的衍射影响，但实际可获得的分辨率还受到光学像差的限制。在过去二十年中，超分辨率显微镜应运而生，它克服了衍射障碍，能够在纳米尺度上进行大量生物学研究。自适应光学技术借鉴自天文成像领域，在几乎每种显微镜模态中都已被应用于校正光学像差，尤其是在过去十年的超分辨率显微镜中，以恢复最佳图像质量和分辨率。在本综述中，我们简要介绍自适应光学的基本概念以及主要超分辨率成像技术的工作原理。我们重点介绍了自适应光学在超分辨率显微镜中用于主动和动态像差校正的一些最新应用和进展。
