Implementation of ka-Based Strategy for Scaling Up (Red Marine Microalga) to Produce High-Value Phycoerythrin, Fatty Acids, and Proteins.

is a well-known Rhodophyta that recently has attracted enormous attention because of its capacity to produce many high-value metabolites such as the pigment phycoerythrin and several high-value fatty acids. Phycoerythrin is a fluorescent red protein-pigment commercially relevant with antioxidant, antimicrobial activity, and fluorescent properties. The volumetric mass transfer coefficient (ka) was kept constant within the different scaling-up stages in the present study. This scaling-up strategy was sought to maintain phycoerythrin production and other high-value metabolites by , using hanging-bag photobioreactors. The ka was monitored to ensure the appropriate mixing and CO diffusion in the entire culture during the scaling process (16, 80, and 400 L). Then, biomass concentration, proteins, fatty acids, carbohydrates, and phycoerythrin were determined in each step of the scaling-up process. The ka at 16 L reached a level of 0.0052 s, while at 80 L, a value of 0.0024 s was achieved. This work result indicated that at 400 L, 1.22 g L of biomass was obtained, and total carbohydrates (117.24 mg L), proteins (240.63 mg L), and lipids (17.75% DW) were accumulated. Regarding fatty acids production, 46.03% palmitic, 8.03% linoleic, 22.67% arachidonic, and 2.55% eicosapentaenoic acid were identified, principally. The phycoerythrin production was 20.88 mg L with a purity of 2.75, making it viable for food-related applications. The results of these experiments provide insight into the high-scale production of phycoerythrin via the cultivation of in an inexpensive and straightforward culture system.