University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Bioresour Technol. 2013 Nov;147:160-167. doi: 10.1016/j.biortech.2013.08.051. Epub 2013 Aug 13.
Microalgae have emerged as promising resources for highly unsaturated fatty acids. In this study, an indigenous microalga identified as Nannochloropsis oceanica CY2 was grown photoautotrophically to produce eicosapentaenoic acid (EPA; 20:5, n-3). Specific engineering strategies were employed to stimulate EPA accumulation in the microalgal cells. The results show that BG-11 was the most effective medium to grow N. oceanica CY2, giving an EPA content and biomass concentration of 2.38% (per dry cell weight) and 1.53 g/l. The EPA content nearly doubled when using the optimal nitrogen source (NaNO3) at a concentration of 1.50 g/l. The illumination system also markedly affected the EPA content for the photoautotrophic microalga. When the microalgal culture was illuminated with a red LED, an impressively high EPA content of 5.5% was obtained. Finally, using semi-batch cultures operations with LED-blue illumination, the EPA content of N. oceanica CY2 was stably maintained at 5.0%.
微藻已成为高度不饱和脂肪酸的有前途的资源。在这项研究中，一种被鉴定为 Nannochloropsis oceanica CY2 的本土微藻通过光自养生长来生产二十碳五烯酸 (EPA；20:5，n-3)。采用特定的工程策略来刺激微藻细胞中 EPA 的积累。结果表明，BG-11 是生长 N. oceanica CY2 的最有效培养基，给出了 2.38%（干细胞重量）和 1.53 g/l 的 EPA 含量和生物质浓度。当使用最佳氮源 (NaNO3) 浓度为 1.50 g/l 时，EPA 含量几乎翻了一番。光照系统也显著影响光自养微藻的 EPA 含量。当微藻培养在红色 LED 下进行光照时，可获得令人印象深刻的高 EPA 含量 5.5%。最后，使用具有 LED 蓝色照明的半分批培养操作，可稳定地将 N. oceanica CY2 的 EPA 含量维持在 5.0%。
