Wang Ying, Yu Hongxiang, Chen Ying, Li Gang, Lei Ying, Zhao Jian
Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Institutes of Biomedical Sciences & Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Stem Cell Res. 2018 Aug;31:127-130. doi: 10.1016/j.scr.2018.07.018. Epub 2018 Jul 25.
A 87-year old Alzheimer's Disease(AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.
一名87岁的阿尔茨海默病（AD）男性患者捐赠了他的外周血单个核细胞（PBMC）。使用非整合型附加体质粒载体系统，用人OKSM转录因子对PBMC进行重编程。通过对多能性标志物的免疫细胞化学以及iPSC在体外自发分化为三个胚层的能力，证实了无转基因iPSC的多能性。此外，该iPSC系显示出正常的核型。我们的模型可能为进一步研究病理机制、识别早期生物标志物以及进行AD药物测试研究提供一个良好的平台。
