Mohri Shinsuke, Takahashi Haruya, Sakai Maiko, Takahashi Shingo, Waki Naoko, Aizawa Koichi, Suganuma Hiroyuki, Ara Takeshi, Matsumura Yasuki, Shibata Daisuke, Goto Tsuyoshi, Kawada Teruo
Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan.
KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
PLoS One. 2018 Jan 12;13(1):e0191203. doi: 10.1371/journal.pone.0191203. eCollection 2018.
Obesity-induced chronic inflammation is a key factor in type 2 diabetes. A vicious cycle involving pro-inflammatory mediators between adipocytes and macrophages is a common cause of chronic inflammation in the adipose tissue. Tomato is one of the most popular vegetables and is associated with a reduced risk of diabetes. However, the molecular mechanism underlying the effect of tomato on diabetes is unclear. In this study, we focused on anti-inflammatory compounds in tomato. We found that the extract of tomato reduced plasma glucose and inflammatory markers in mice. We screened anti-inflammatory fractions in tomato using lipopolysaccharide-stimulated RAW264.7 macrophages, and active compounds were estimated by liquid chromatography-mass spectrometry over a wide range. Surprisingly, a large number of compounds including oxylipin and coumarin derivatives were estimated as anti-inflammatory compounds. Especially, 9-oxo-octadecadienoic acid and daphnetin suppressed pro-inflammatory cytokines in RAW264.7 macrophages inhibiting mitogen-activated protein kinase phosphorylation and inhibitor of kappa B α protein degradation. These findings suggest that tomato containing diverse anti-inflammatory compounds ameliorates chronic inflammation in obese adipose tissue.
肥胖诱导的慢性炎症是2型糖尿病的关键因素。脂肪细胞和巨噬细胞之间涉及促炎介质的恶性循环是脂肪组织慢性炎症的常见原因。番茄是最受欢迎的蔬菜之一，与降低糖尿病风险有关。然而，番茄对糖尿病影响的分子机制尚不清楚。在本研究中，我们聚焦于番茄中的抗炎化合物。我们发现番茄提取物可降低小鼠的血糖和炎症标志物。我们使用脂多糖刺激的RAW264.7巨噬细胞筛选番茄中的抗炎组分，并通过液相色谱-质谱在较宽范围内评估活性化合物。令人惊讶的是，大量包括氧化脂质和香豆素衍生物在内的化合物被评估为抗炎化合物。特别是，9-氧代-十八碳二烯酸和瑞香素抑制RAW264.7巨噬细胞中的促炎细胞因子，抑制丝裂原活化蛋白激酶磷酸化和κBα蛋白降解。这些发现表明，含有多种抗炎化合物的番茄可改善肥胖脂肪组织中的慢性炎症。
