Department of Chemical Engineering, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China; Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA.
Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA.
Chin J Nat Med. 2017 Nov;15(11):816-824. doi: 10.1016/S1875-5364(18)30015-3.
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme (IC of 0.4-1.4 μg·mL) than the pharmaceutical acarbose (IC 141.8 μg·mL). B6 and B7 (IC 17.6 and 11.7 μg·mL, respectively) exhibited α-amylase inhibitory activity as efficacious as acarbose (IC 15.4 μg·mL). Moreover, B extract, at 25 µg·mL, significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.
本研究旨在对金合欢树皮粗提物（B）及其高速逆流色谱（HSCCC）分离得到的馏分（B1-B7）中的多酚进行表征，并评估其抗炎和糖分解酶（α-葡萄糖苷酶和α-淀粉酶）抑制活性。馏分 B4、B5、B6、B7（总酚含量分别为 850.3、983.0、843.9 和 572.5 mg·g；原花青素含量分别为 75.7、90.5、95.0 和 44.8 mg·g）对脂多糖（LPS）刺激的小鼠巨噬细胞 RAW 264.7 细胞系中的活性氧（ROS）、一氧化氮（NO）产生和促炎基因白细胞介素-1β（IL-1β）和诱导型一氧化氮合酶（iNOS）的表达具有显著的抑制作用。所有提取物均抑制α-葡萄糖苷酶和α-淀粉酶的活性，这两种酶是碳水化合物消化的主要酶。金合欢树皮样品对α-葡萄糖苷酶（IC 0.4-1.4 μg·mL）的抑制作用明显强于药物阿卡波糖（IC 141.8 μg·mL）。B6 和 B7（IC 17.6 和 11.7 μg·mL，分别）对α-淀粉酶的抑制活性与阿卡波糖（IC 15.4 μg·mL）相当。此外，B 提取物在 25 µg·mL 时，可显著降低与人类单核巨噬细胞炎症反应相关的非线粒体氧化爆发。
