Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Food Chem. 2018 May 15;248:70-77. doi: 10.1016/j.foodchem.2017.12.040. Epub 2017 Dec 13.
The aim of this work was the development of a simple, novel and accurate method for the determination of adenosine triphosphate (ATP) and its first five catabolites: adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophosphate (IMP), inosine (Ino) and hypoxanthine (Hx), in fish tissue, based on hydrophilic interaction liquid chromatography (HILIC). For this purpose, a stationary phase for polar and hydrophilic compounds (ZIC-pHILIC) was used. The effect of different chromatographic parameters and the molecular mechanism based on the van't Hoff plot were examined. The t-test and Dixon's Q-test were applied in order to examine statistical differences and outlier values. The recovery of the method ranged between 82.7% and 127% and the %RSD values were lower than 10% for all analytes determined. The method was applied in frozen sea bream samples stored at 0-4 °C. The K-, G-, H- and F values were calculated for the estimation of the level of fish freshness.
本工作旨在开发一种简单、新颖且准确的方法，用于测定鱼类组织中的三磷酸腺苷 (ATP) 及其前五种分解产物：二磷酸腺苷 (ADP)、一磷酸腺苷 (AMP)、次黄苷单磷酸 (IMP)、次黄嘌呤核苷 (Ino) 和黄嘌呤 (Hx)，该方法基于亲水相互作用液相色谱 (HILIC)。为此，使用了一种用于极性和亲水化合物的固定相 (ZIC-pHILIC)。考察了不同色谱参数的影响以及基于范特霍夫图的分子机制。为了检验统计差异和异常值，应用了 t 检验和 Dixon 的 Q 检验。该方法的回收率在 82.7%至 127%之间，所有测定物的 %RSD 值均低于 10%。该方法应用于在 0-4°C 下储存的冷冻海鲈样品中。计算了 K、G、H 和 F 值，以估计鱼类新鲜度水平。
