Burns B G, Ke P J
J Assoc Off Anal Chem. 1985 May-Jun;68(3):444-8.
A liquid chromatography (LC) method for determining the hypoxanthine content in fish tissues has been developed. Hypoxanthine is extracted with 0.6M perchloric acid, and determined by LC on a reverse phase microparticulate column with UV absorbance detection. The mobile phase is 0.01M potassium phosphate buffer (pH 4.5). The percent relative standard deviation for measurements by the recommended method was less than 7% with a detection limit of 10 ng. Recoveries of hypoxanthine added to various fish tissues were better than 90%. The operational errors, interferences, and recoveries for spiked samples have been investigated and compare favorably with an established xanthine oxidase enzyme method. The described LC method is simple, rapid, and specific for measuring hypoxanthine content in various fish tissues. Some post-mortem studies have indicated the method may also be used for the determination of adenosine monophosphate, inosine monophosphate, and inosine.
已开发出一种用于测定鱼类组织中次黄嘌呤含量的液相色谱（LC）方法。次黄嘌呤用0.6M高氯酸提取，并通过LC在反相微粒柱上进行紫外吸光度检测来测定。流动相为0.01M磷酸钾缓冲液（pH 4.5）。推荐方法测量的相对标准偏差百分比小于7%，检测限为10 ng。添加到各种鱼类组织中的次黄嘌呤回收率优于90%。对加标样品的操作误差、干扰和回收率进行了研究，并与已建立的黄嘌呤氧化酶法进行了比较，结果良好。所描述的LC方法简单、快速且特异性强，可用于测量各种鱼类组织中的次黄嘌呤含量。一些死后研究表明，该方法也可用于测定一磷酸腺苷、一磷酸肌苷和肌苷。
