Division of Medicine, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
Division of Medicine, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
Microb Pathog. 2018 Mar;116:33-37. doi: 10.1016/j.micpath.2018.01.001. Epub 2018 Jan 9.
Bovine respiratory disease (BRD) is one of the leading causes of morbidity and mortality in dairy calves. Identification of reliable biomarkers of naturally occurring BRD is essential for ensuring early diagnosis and treatment of calves and monitoring treatment efficacy. This need is punctuated, especially in mild to moderate cases that would greatly help to decrease recurrence and the overall prevalence of BRD. The present study was conducted to investigate the changes in serum concentrations of haptoglobin (Hpt) and serum amyloid A (SAA) and association between oxidative stress and acute phase proteins (APPs) in BRD. Hpt and SAA levels significantly increased (P < .01) in BRD stressed calves as compared to healthy subjects. There was a significant decrease (P < .01) in serum albumin (Alb) concentration of infected calves as compared to controls. The oxidative stress markers revealed a significant (P < .01) increase in lipid peroxidation (LPO) and a concurrent decrease in activities of superoxide dismutase (SOD), reduced glutathione (R-GSH) and catalase (CAT) in BRD. A significant correlation among APPs, extent of oxidative stress and clinical score (CS) of calves was depicted. A stepwise decrease in Hpt and SAA and increase in Alb was observed in infected calves post-treatment. These results suggest implication of oxidative stress in enhancing APPs and monitoring of APPs as a potential complement to clinical assessment of treatment in calves with naturally occurring BRD. Hpt may be useful as the most sensitive biomarker in BRD. However, the combined use of Hpt and oxidative stress biomarkers would greatly improve the diagnostic accuracy.
牛呼吸道疾病（BRD）是导致奶牛犊牛发病率和死亡率的主要原因之一。鉴定出自然发生的 BRD 的可靠生物标志物对于确保对犊牛进行早期诊断和治疗以及监测治疗效果至关重要。这种需求非常迫切，尤其是在轻度至中度病例中，这将极大地有助于降低 BRD 的复发率和总体流行率。本研究旨在探讨 BRD 中血清结合珠蛋白（Hpt）和血清淀粉样蛋白 A（SAA）浓度的变化以及氧化应激与急性相蛋白（APPs）之间的关系。与健康对照组相比，BRD 应激犊牛的 Hpt 和 SAA 水平显著升高（P<.01）。与对照组相比，感染犊牛的血清白蛋白（Alb）浓度显著降低（P<.01）。氧化应激标志物显示脂质过氧化（LPO）显著增加（P<.01），同时超氧化物歧化酶（SOD）、还原型谷胱甘肽（R-GSH）和过氧化氢酶（CAT）的活性降低（P<.01）。APPs、氧化应激程度和犊牛临床评分（CS）之间存在显著相关性。感染犊牛在治疗后观察到 Hpt 和 SAA 水平逐渐降低，Alb 水平逐渐升高。这些结果表明，氧化应激在增强 APPs 中的作用，以及监测 APPs 作为对自然发生的 BRD 治疗进行临床评估的潜在补充，可能具有重要意义。Hpt 可能是 BRD 中最敏感的生物标志物。然而，Hpt 和氧化应激生物标志物的联合使用将极大地提高诊断准确性。
