Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, 32827, USA.
Sci Rep. 2018 Jan 12;8(1):675. doi: 10.1038/s41598-017-18945-z.
Head and neck cancer is characterized by malignant tumors arising from the epithelium covering the upper aerodigestive tract, and the majority of these epithelial malignancies are squamous cell carcinomas (SCCs) of the oral cavity (OSCCs). The aim of the current work was to identify miRNAs regulated in OSCC cancerous tissue when compared to a healthy adjacent tissue and to verify the presence of the same miRNAs in the circulation of these patients. For that serum samples and biopsies of healthy and tumor tissues were collected from five patients diagnosed with OSCC of the oral cavity, RNA was extracted from these samples and microRNAs libraries were prepared and sequenced. A total 255 miRNAs were identified in tissue and 381 different miRNAs were identified in serum samples. When comparing the miRNA expression between tumor and healthy tissue we identified 48 miRNAs (25 down- and 23 up-regulated) that were differentially expressed (FDR < 0.05). From these 48 differentially expressed miRNAs in tissue, 30 miRNAs were also found in the serum of the same patients. hsa-miR-32-5p was up-regulated in tumor compared to healthy tissue in our study, and was previously shown to be up-regulated in the serum of OSCC patients. Therefore, this suggests that miRNAs can be used as potential non-invasive biomarkers of OSCC.
头颈部癌症的特征是来源于覆盖上呼吸道的上皮组织的恶性肿瘤，这些上皮恶性肿瘤大多数为口腔的鳞状细胞癌（OSCC）。目前这项工作的目的是确定与健康相邻组织相比，口腔鳞状细胞癌癌组织中受调控的 miRNA，并验证这些患者的循环中是否存在相同的 miRNA。为此，从 5 名被诊断为口腔 OSCC 的患者中收集了血清样本和健康及肿瘤组织的活检，从这些样本中提取 RNA，并准备和测序 microRNA 文库。在组织中鉴定出了 255 种 miRNA，在血清样本中鉴定出了 381 种不同的 miRNA。当比较肿瘤和健康组织中的 miRNA 表达时，我们确定了 48 个 miRNA（25 个下调和 23 个上调）存在差异表达（FDR<0.05）。在组织中这 48 个差异表达的 miRNA 中，有 30 个也在同一患者的血清中被发现。在我们的研究中，hsa-miR-32-5p 在肿瘤中相对于健康组织上调，并且先前在 OSCC 患者的血清中显示上调。因此，这表明 miRNA 可以作为 OSCC 的潜在非侵入性生物标志物。
