Coordes Stefanie Louise, Jost-Brinkmann Paul-Georg, Präger Thomas Michael, Bartzela Theodosia, Visel Dominik, Jäcker Theresa, Müller-Hartwich Ralf
Department of Orthodontics, Dentofacial Orthopedics and Pedodontics, Center for Dental and Craniofacial Sciences, Charité - Universitätsmedizin Berlin, Aßmannshauser Straße 4-6, 14197, Berlin, Germany.
J Orofac Orthop. 2018 Jan;79(1):49-56. doi: 10.1007/s00056-017-0116-y. Epub 2018 Jan 12.
Aim of the study was to compare how six different sealants resisted thermal, mechanical, and chemical loading in vitro.
In all, 120 extracted human, nondecayed molars were divided into six groups (20 samples each) and embedded in resin blocks. The buccal surfaces of the tooth samples were polished and divided into three areas. Area A contained the product to be analyzed, area B was covered with colorless nail varnish (negative control), and area C remained untreated (positive control). The samples were stored in 0.1% thymol solution. To simulate a 3-month thermomechanical load, the samples were subjected to thermal cycling and a cleaning device. After 7 days incubation in a ten Cate demineralization solution (pH value: 4.6), the samples were dissected using a band saw and the lesion depths and demineralization areas were evaluated and compared microscopically.
The tooth surfaces treated with PRO SEAL® showed no demineralization. Mean lesion depths of 108.1, 119.9, 154.9, 149.2, and 184.5 μm were found with Alpha-Glaze®, Seal&Protect®, Tiefenfluorid®, Protecto®, and Fluor Protector, respectively. There was a significant difference between PRO SEAL® and the other products (p > 0.0001). There was no significant difference between the other products.
PRO SEAL® resisted thermal, mechanical, and chemical loading in vitro, providing protection against white spot lesions.
本研究旨在比较六种不同的封闭剂在体外对热、机械和化学负荷的抵抗能力。
总共120颗拔除的人类非龋磨牙被分为六组（每组20个样本），并嵌入树脂块中。牙齿样本的颊面被抛光并分为三个区域。区域A包含待分析的产品，区域B覆盖无色指甲油（阴性对照），区域C未作处理（阳性对照）。样本储存在0.1%的百里酚溶液中。为模拟3个月的热机械负荷，样本经历热循环和一个清洁装置处理。在ten Cate脱矿溶液（pH值：4.6）中孵育7天后，使用带锯对样本进行切割，通过显微镜评估并比较病变深度和脱矿区域。
用PRO SEAL®处理的牙齿表面未出现脱矿。使用Alpha-Glaze®、Seal&Protect®、Tiefenfluorid®、Protecto®和Fluor Protector处理后的平均病变深度分别为108.1、119.9、154.9、149.2和184.5μm。PRO SEAL®与其他产品之间存在显著差异（p > 0.0001）。其他产品之间无显著差异。
PRO SEAL®在体外能抵抗热、机械和化学负荷，可预防白斑病变。
