Yang Yong-Jie, Liu Zeng-Shan, Lu Shi-Ying, Hu Pan, Li Chuang, Ahmad Waqas, Li Yan-Song, Xu Yun-Ming, Tang Feng, Zhou Yu, Ren Hong-Lin
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Department of Food Science, College of Agriculture, Yanbian University, Yanji 133002, China.
J Vet Res. 2018 Mar 30;62(1):113-119. doi: 10.1515/jvetres-2018-0016. eCollection 2018 Mar.
Serological diagnosis of brucellosis is still a great challenge due to the infeasibility of discriminating infected animals from vaccinated ones, so it is necessary to search for diagnostic biomarkers for differential diagnosis of brucellosis.
Cell division cycle 42 (Cdc42) from sheep () (OaCdc42) was cloned by rapid amplification of cDNA ends (RACE), and then tissue distribution and differential expression levels of OaCdc42 mRNA between infected and vaccinated sheep were analysed by RT-qPCR.
The full-length cDNA of OaCdc42 was 1,609 bp containing an open reading frame (ORF) of 576 bp. OaCdc42 mRNAs were detected in the heart, liver, spleen, lung, kidneys, rumen, small intestine, skeletal muscles, and buffy coat, and the highest expression was detected in the small intestine. Compared to the control, the levels of OaCdc42 mRNA from sheep infected with or sheep vaccinated with S2 was significantly different (P < 0.01) after 40 and 30 days post-inoculation, respectively. However, the expression of OaCdc42 mRNA was significantly different between vaccinated and infected sheep (P < 0.05 or P < 0.01) on days: 14, 30, and 60 post-inoculation, whereas no significant difference (P > 0.05) was noted 40 days post-inoculation. Moreover, the expression of OaCdc42 from both infected and vaccinated sheep showed irregularity.
OaCdc42 is not a good potential diagnostic biomarker for differential diagnosis of brucellosis in sheep.
由于难以区分感染动物和接种疫苗的动物，布鲁氏菌病的血清学诊断仍然是一项巨大挑战，因此有必要寻找用于布鲁氏菌病鉴别诊断的诊断生物标志物。
通过cDNA末端快速扩增（RACE）克隆绵羊（Ovis aries）的细胞分裂周期42（Cdc42）（OaCdc42），然后通过RT-qPCR分析OaCdc42 mRNA在感染和接种疫苗的绵羊之间的组织分布和差异表达水平。
OaCdc42的全长cDNA为1609 bp，包含一个576 bp的开放阅读框（ORF）。在心脏、肝脏、脾脏、肺、肾脏、瘤胃、小肠、骨骼肌和血沉棕黄层中检测到OaCdc42 mRNA，在小肠中表达最高。与对照组相比，感染布鲁氏菌或接种S2疫苗的绵羊在接种后40天和30天，OaCdc42 mRNA水平分别有显著差异（P < 0.01）。然而，在接种后第14、30和60天，接种疫苗和感染的绵羊之间OaCdc42 mRNA的表达有显著差异（P < 0.05或P < 0.01），而在接种后40天无显著差异（P > 0.05）。此外，感染和接种疫苗的绵羊中OaCdc42的表达均显示出不规则性。
OaCdc42不是用于绵羊布鲁氏菌病鉴别诊断的良好潜在诊断生物标志物。
