Institute of Nephrology, Zhong Da Hospital, Southeast University, School of Medicine, Nanjing, Jiangsu, 210009, China.
Institute of Nephrology, Zhong Da Hospital, Southeast University, School of Medicine, Nanjing, Jiangsu, 210009, China.
Mol Cell Endocrinol. 2018 Sep 15;473:44-52. doi: 10.1016/j.mce.2018.01.001. Epub 2018 Jan 10.
This study intends to investigate the effect of active vitamin D (VD) on the expression of triggering receptor expressed on myeloid cells-1 (TREM-1) in the renal tissues of diabetic nephropathy (DN) rats and to explore the impact of TREM-1 on macrophage adhesion and migration. We find that the expressions of TREM-1 and CD68 protein are higher in DN rats compared with rats in the normal control group and that these changes are decreased in the DN + VD group. In vitro, the capacity for macrophage adhesion and migration and the expression of TREM-1 are increased under high-glucose conditions, but VD inhibits this progress. TREM-1 siRNA decreases high-glucose-induced macrophage adhesion and migration, whereas over-expression of TREM-1 inhibits its action. However, VD cannot suppress high glucose-induced TREM-1 expression and macrophage adhesion and migration when TREM-1 is over-expressed. These results demonstrate that VD can suppress macrophage adhesion and migration by reducing the expression of TREM-1.
本研究旨在探讨活性维生素 D（VD）对糖尿病肾病（DN）大鼠肾组织中髓系细胞触发受体-1（TREM-1）表达的影响，并探讨 TREM-1对巨噬细胞黏附和迁移的影响。我们发现，与正常对照组大鼠相比，DN 大鼠的 TREM-1 和 CD68 蛋白表达更高，而在 DN+VD 组这些变化则减少。在体外，高糖条件下巨噬细胞黏附和迁移的能力以及 TREM-1 的表达增加，但 VD 抑制了这一过程。TREM-1 siRNA 降低高糖诱导的巨噬细胞黏附和迁移，而过表达 TREM-1 则抑制其作用。然而，当 TREM-1 过表达时，VD 不能抑制高糖诱导的 TREM-1 表达和巨噬细胞黏附和迁移。这些结果表明，VD 可以通过降低 TREM-1 的表达来抑制巨噬细胞的黏附和迁移。
